Andreadelli Aggeliki, Petrakis Spyros, Tsoureki Antiopi, Tsiolas George, Michailidou Sofia, Baltzopoulou Penelope, Merkestein Robert van, Hodgson Philip, Sceats Mark, Karagiannakis George, Makris Antonios M
Institute of Applied Biosciences, Centre for Research & Technology, Hellas (CERTH), 570 01 Thessaloniki, Greece.
Chemical Process & Energy Resources Institute, Centre for Research & Technology, Hellas (CERTH), 570 01 Thessaloniki, Greece.
Microorganisms. 2021 Jun 4;9(6):1217. doi: 10.3390/microorganisms9061217.
Recently, metal oxides and magnesium hydroxide nanoparticles (NPs) with high surface-to-volume ratios were shown to possess antibacterial properties with applications in biomedicine and agriculture. To assess recent observations from field trials on tomatoes showing resistance to pathogen attacks, porous micron-scale particles composed of nano-grains of MgO were hydrated and sprayed on the leaves of healthy tomato () plants in a 20-day program. The results showed that the spray induced (a) a modest and selective stress gene response that was consistent with the absence of phytotoxicity and the production of salicylic acid as a signalling response to pathogens; (b) a shift of the phylloplane microbiota from near 100% dominance by Gram (-) bacteria, leaving extremophiles and cyanobacteria to cover the void; and (c) a response of the fungal leaf phylloplane that showed that the leaf epiphytome was unchanged but the fungal load was reduced by about 70%. The direct microbiome changes together with the low level priming of the plant's immune system may explain the previously observed resistance to pathogen assaults in field tomato plants sprayed with the same hydrated porous micron-scale particles.
最近，具有高比表面积的金属氧化物和氢氧化镁纳米颗粒（NPs）被证明具有抗菌特性，可应用于生物医学和农业领域。为了评估最近对番茄进行的田间试验观察结果，这些试验表明番茄对病原体攻击具有抗性，将由纳米级氧化镁颗粒组成的多孔微米级颗粒进行水合处理，并在一个为期20天的计划中喷洒在健康番茄植株的叶片上。结果表明，喷雾诱导了：（a）适度且具有选择性的应激基因反应，这与无植物毒性以及作为对病原体信号反应的水杨酸产生相一致；（b）叶际微生物群从革兰氏阴性菌近乎100%的优势地位发生转变，留下嗜极菌和蓝细菌来填补空白；（c）真菌叶际的反应表明，叶附生植物群落未发生变化，但真菌载量降低了约70%。直接的微生物群落变化以及植物免疫系统的低水平启动可能解释了之前在用相同水合多孔微米级颗粒喷洒的田间番茄植株中观察到的对病原体攻击的抗性。
