Centre for Agriculture and the Bioeconomy (CAB), Queensland University of Technology (QUT), Brisbane, QLD 4001, Australia.
Department of Agriculture and Fisheries (DAF), Maroochy Research Facility, Nambour, QLD 4560, Australia.
Viruses. 2021 Jun 4;13(6):1071. doi: 10.3390/v13061071.
We have characterized the complete genome sequence of an Australian isolate of banana streak CA virus (BSCAV). A greater-than-full-length, cloned copy of the virus genome was assembled and agroinoculated into five tissue-cultured plants of nine different banana accessions. BSCAV was highly infectious in all nine accessions. All five inoculated plants from eight accessions developed symptoms by 28 weeks post-inoculation, while all five plants of AA subsp. remained symptomless. Symptoms were mild in six accessions but were severe in Khae Phrae (. subsp. ) and the East African Highland banana accession Igisahira Gisanzwe. This is the first full-length BSCAV genome sequence reported from Australia and the first report of the infectivity of an infectious clone of banana streak virus.
我们已经对澳大利亚蕉条 CA 病毒(BSCAV)的一个分离株的全基因组序列进行了特征描述。该病毒的一个大于全长的克隆拷贝被组装并通过农杆菌接种到 9 种不同的香蕉品种的 5 个组织培养植株中。BSCAV 在所有 9 个品种中都具有高度传染性。在接种后 28 周,来自 8 个品种的所有 5 株植物都出现了症状,而 AA 亚种的所有 5 株植物均无症状。6 个品种的症状较轻微,但在 Khae Phrae(. 亚种)和东非高地香蕉品种 Igisahira Gisanzwe 中症状较严重。这是首次从澳大利亚报道的完整 BSCAV 基因组序列,也是首次报道香蕉条纹病毒的传染性克隆的感染性。