Institute of Natural Science, Yeungnam Univiersity, Gyeongsan-si 38541, Gyeongbuk-do, Korea.
Department of Life Sciences, Yeungnam University, Gyeongsan-si 38541, Gyeongbuk-do, Korea.
Int J Mol Sci. 2021 Jun 7;22(11):6143. doi: 10.3390/ijms22116143.
Orobanchaceae have become a model group for studies on the evolution of parasitic flowering plants, and , a holoparasitic plant, is a member of this family. In this study, we assembled the complete chloroplast and mitochondrial genomes of . The chloroplast and mitochondrial genomes were 56,381 bp and 401,628 bp long, respectively. The chloroplast genome of shows massive plastid genes and the loss of one IR (inverted repeat). A comparison of the chloroplast genome sequence with that of a previous study demonstrated that the two chloroplast genomes encode a similar number of proteins (except ) but differ greatly in length. The mitochondrial genome has 53 genes, including 35 protein-coding genes (34 native mitochondrial genes and one chloroplast gene), 15 tRNA (11 native mitochondrial genes and four chloroplast genes) genes, and three rRNA genes. Evidence for intracellular gene transfer (IGT) and horizontal gene transfer (HGT) was obtained for plastid and mitochondrial genomes. ψ and ψ in the mitogenome were transferred from the plastid genome of . The gene in the plastid of was transferred from another plastid angiosperm plastid and the gene in mitogenome was transferred from a host plant like . () encodes proteins containing a membrane domain, making ORF (Open Reading Frame) the most likely candidate gene for CMS development in .
列当科已成为研究寄生开花植物进化的模式类群，而列当属的全寄生植物 是该科的成员之一。在这项研究中，我们组装了 的完整叶绿体和线粒体基因组。叶绿体和线粒体基因组分别长 56381bp 和 401628bp。 的叶绿体基因组显示出大量的质体基因和一个 IR（反向重复）的缺失。与之前的研究相比，对 叶绿体基因组序列的比较表明，两个叶绿体基因组编码相似数量的蛋白质（除 外），但长度差异很大。 的线粒体基因组有 53 个基因，包括 35 个蛋白编码基因（34 个原生线粒体基因和一个叶绿体基因）、15 个 tRNA（11 个原生线粒体基因和 4 个叶绿体基因）基因和 3 个 rRNA 基因。对质体和线粒体基因组的细胞内基因转移（IGT）和水平基因转移（HGT）进行了研究。 线粒体基因组中的 ψ 和 ψ 是从 的质体基因组转移而来的。 质体中的 基因是从另一个质体被子植物转移而来的，而 线粒体基因组中的 基因是从宿主植物 转移而来的。 () 编码含有膜结构域的蛋白质，使得 ORF（开放阅读框）成为 中 CMS 发育的最可能候选基因。
