Rizzardi Nicola, Liparulo Irene, Antonelli Giorgia, Orsini Francesca, Riva Antonella, Bergamini Christian, Fato Romana
Department of Pharmacy and Biotechnology, FABIT, University of Bologna, 6, 40126 Bologna, Italy.
Indena SpA, Viale Ortles, 20139 Milan, Italy.
Antioxidants (Basel). 2021 Jun 7;10(6):927. doi: 10.3390/antiox10060927.
Coenzyme Q10 (CoQ10) is a lipid-soluble molecule with a dual role: it transfers electrons in the mitochondrial transport chain by promoting the transmembrane potential exploited by the ATPase to synthesize ATP and, in its reduced form, is a membrane antioxidant. Since the high CoQ10 hydrophobicity hinders its bioavailability, several formulations have been developed to facilitate its cellular uptake. In this work, we studied the bioenergetic and antioxidant effects in I407 and H9c2 cells of a CoQ10 phytosome formulation (UBIQSOME, UBQ). We investigated the cellular and mitochondrial content of CoQ10 and its redox state after incubation with UBQ. We studied different bioenergetic parameters, such as oxygen consumption, ATP content and mitochondrial potential. Moreover, we evaluated the effects of CoQ10 incubation on oxidative stress, membrane lipid peroxidation and ferroptosis and highlighted the connection between the intracellular concentration of CoQ10 and its antioxidant potency. Finally, we focused on the cellular mechanism that regulates UBQ internalization. We showed that the cell lines used in this work share the same uptake mechanism for UBQ, although the intestinal cell line was less efficient. Given the limitations of an in vitro model, the latter result supports that intestinal absorption is a critical step for the oral administration of Coenzyme Q10 formulations.
辅酶Q10（CoQ10）是一种具有双重作用的脂溶性分子：它通过促进ATP酶利用跨膜电位来合成ATP，从而在线粒体运输链中传递电子，并且其还原形式是一种膜抗氧化剂。由于CoQ10的高疏水性阻碍了其生物利用度，因此已经开发了几种制剂来促进其细胞摄取。在这项工作中，我们研究了CoQ10磷脂体制剂（UBIQSOME，UBQ）对I407和H9c2细胞的生物能量和抗氧化作用。我们研究了与UBQ孵育后CoQ10的细胞和线粒体含量及其氧化还原状态。我们研究了不同的生物能量参数，如氧气消耗、ATP含量和线粒体电位。此外，我们评估了CoQ10孵育对氧化应激、膜脂质过氧化和铁死亡的影响，并强调了CoQ10细胞内浓度与其抗氧化能力之间的联系。最后，我们专注于调节UBQ内化的细胞机制。我们表明，尽管肠道细胞系效率较低，但本工作中使用的细胞系对UBQ具有相同的摄取机制。鉴于体外模型的局限性，后一结果支持肠道吸收是辅酶Q10制剂口服给药的关键步骤。
