Barra Ruan Henrique Delmonica, Piovezan Bianca Rafaeli, Matheus Henrique Rinaldi, Vitória Otávio Augusto Pacheco, de Abreu Furquim Elisa Mara, Fiorin Luiz Guilherme, Santos Ester Oliveira, de Almeida Juliano Milanezi
Division of Periodontics, Department of Diagnosis and Surgery, UNESP, São Paulo State University "Júlio de Mesquita Filho", Araçatuba, Brazil.
Nucleus of Study and Research in Periodontics and Implantology (NEPPI), School of Dentistry, Sao Paulo, State University (Unesp), Aracatuba, SP, Brazil.
PLoS One. 2025 Jan 3;20(1):e0315462. doi: 10.1371/journal.pone.0315462. eCollection 2025.
The study aimed to evaluate the potential protection against fractures of oral Q10 supplementation in the tibias of rats exposed to nicotine. Nicotine is known to negatively impact bone density and increase the risk of fractures, in addition to affecting other systems such as the gastrointestinal system, impairing its absorption capacity, negatively affecting bone health. To investigate this, eighty male rats were divided into four groups (n = 20) receiving either nicotine hemisulfate or saline solution (SS) for 28 days. Two daily subcutaneous applications were administered accordingly. Concurrently, vegetable glycerin and Q10 gavage began on day "0". SS: the animals in this group received two daily subcutaneous applications of sodium chloride solution during the entire trial period. 30 days after starting the SS applications subcutaneously, the animals received vegetable glycerin daily until the end of the experiment. SS-Q10: the animals received the SS protocol and daily supplementation with Q10 until the end of the experiment. NIC: The animals received the protocol for NIC and vegetable glycerin daily until the end of the experiment. NIC-Q10: The animals received the protocol for NIC and daily supplementation and Q10 until the end of the experiment. Euthanasia occurred at 7 and 28 days after the beginning the gavage. The tibiae collected were processed for morphometric, densitometric, mechanical, and microtomographic (micro-Ct) analysis. A complementary analysis of intestinal changes was performed. The groups that received Q10 showed slightly better results regarding the mechanical resistance and micro-Ct parameters and to intestinal histomorphometry, as compared with groups not supplemented with Q10. Thus, in rats, it can be concluded that coenzyme Q10 exhibited a protective property to the skeletal system and the gastrointestinal tract, even in the presence of nicotine.
该研究旨在评估口服辅酶Q10对暴露于尼古丁的大鼠胫骨骨折的潜在保护作用。众所周知，尼古丁除了影响其他系统如胃肠系统、损害其吸收能力并对骨骼健康产生负面影响外，还会对骨密度产生负面影响并增加骨折风险。为了对此进行研究，80只雄性大鼠被分为四组（每组n = 20），分别接受半硫酸尼古丁或生理盐水（SS）处理28天。相应地，每天进行两次皮下注射。同时，从第“0”天开始进行蔬菜甘油和辅酶Q10灌胃。SS组：该组动物在整个试验期间每天接受两次皮下注射氯化钠溶液。在开始皮下注射SS 30天后，动物每天接受蔬菜甘油直至实验结束。SS-Q10组：动物接受SS方案并每天补充辅酶Q10直至实验结束。NIC组：动物接受NIC方案并每天接受蔬菜甘油直至实验结束。NIC-Q10组：动物接受NIC方案并每天补充辅酶Q10直至实验结束。在开始灌胃后的第7天和第28天实施安乐死。收集的胫骨用于形态学、密度测定、力学和显微断层扫描（显微CT）分析。对肠道变化进行了补充分析。与未补充辅酶Q10的组相比，接受辅酶Q10的组在力学抗性、显微CT参数和肠道组织形态计量学方面的结果略好。因此，在大鼠中可以得出结论，即使存在尼古丁，辅酶Q10对骨骼系统和胃肠道也具有保护作用。
