Department of Biomedical and Environmental Analysis, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wrocław, Poland.
Students Scientific Association, Department of Biomedical and Environmental Analysis, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wrocław, Poland.
Int J Mol Sci. 2021 Jun 7;22(11):6156. doi: 10.3390/ijms22116156.
Quantum dots (QDs) have a broad range of applications in cell biolabeling, cancer treatment, metastasis imaging, and therapeutic drug monitoring. Despite their wide use, relatively little is known about their influence on other molecules. Interactions between QDs and proteins can influence the properties of both nanoparticles and proteins. The effect of mercaptosuccinic acid-capped CdTe QDs on intercellular copper-zinc superoxide dismutase (SOD1)-one of the main enzymatic antioxidants-was investigated. Incubation of SOD1 with QDs caused an increase in SOD1 activity, unlike in the case of CdCl, which inhibited SOD1. Moreover, this effect on SOD1 increased with the size and potential of QDs, although the effect became clearly visible in higher concentrations of QDs. The intensity of QD-SOD1 fluorescence, analyzed with the use of capillary electrophoresis with laser-induced fluorescence detection, was dependent on SOD1 concentration. In the case of green QDs, the fluorescence signal decreased with increasing SOD1 concentration. In contrast, the signal strength for Y-QD complexes was not dependent on SOD1 dilutions. The migration time of QDs and their complexes with SOD1 varied depending on the type of QD used. The migration time of G-QD complexes with SOD1 differed slightly. However, in the case of Y-QD complexes with SOD1, the differences in the migration time were not dependent on SOD concentration. This research shows that QDs interact with SOD1 and the influence of QDs on SOD activity is size-dependent. With this knowledge, one might be able to control the activation/inhibition of specific enzymes, such as SOD1.
量子点 (QDs) 在细胞生物标记、癌症治疗、转移成像和治疗药物监测等方面有广泛的应用。尽管它们被广泛使用，但人们对它们对其他分子的影响知之甚少。QDs 与蛋白质之间的相互作用会影响纳米颗粒和蛋白质的性质。本研究考察了巯基琥珀酸封端的 CdTe QDs 对细胞内铜锌超氧化物歧化酶（SOD1-主要的酶抗氧化剂之一）的影响。与 CdCl 抑制 SOD1 不同，QDs 孵育 SOD1 会导致 SOD1 活性增加。此外，这种对 SOD1 的影响随 QDs 的尺寸和电位增加而增加，尽管在更高浓度的 QDs 下这种影响变得更加明显。使用毛细管电泳激光诱导荧光检测分析了 QD-SOD1 荧光强度，发现其依赖于 SOD1 浓度。对于绿色 QDs，随着 SOD1 浓度的增加，荧光信号减弱。相比之下，Y-QD 复合物的信号强度不依赖于 SOD1 的稀释度。QDs 及其与 SOD1 的复合物的迁移时间取决于所使用的 QD 类型。G-QD 复合物与 SOD1 的迁移时间略有不同。然而，对于 Y-QD 复合物与 SOD1，迁移时间的差异不依赖于 SOD 浓度。本研究表明，QDs 与 SOD1 相互作用，且 QDs 对 SOD 活性的影响是尺寸依赖性的。有了这些知识，人们或许能够控制特定酶（如 SOD1）的激活/抑制。
