Maake Takalani Whitney, Aiyegoro Olayinka Ayobami, Adeleke Matthew Adekunle
Discipline of Genetics, School of Life Sciences, College of Agricultural, Engineering and Science, University of Kwazulu-Natal, Westville Campus, Private Bag X 54001, Durban 4000, South Africa.
Gastrointestinal Microbiology and Biotechnology, Agricultural Research Council-Animal Production, Private Bag X 02, Irene 0062, South Africa.
Vet Sci. 2021 Jun 7;8(6):103. doi: 10.3390/vetsci8060103.
The effects on rumen microbial communities of direct-fed probiotics, and , singly and in combination as feed supplements to both the Boer and Speckled goats were studied using the Illumina Miseq platform targeting the V3-V4 region of the 16S rRNA microbial genes from sampled rumen fluid. Thirty-six goats of both the Boer and Speckled were divided into five experimental groups: (T1) = diet + ; (T2) = diet + ; (T3) = diet + + ; (T4, positive control) = diet + antibiotic and (T5, negative control) = diet without antibiotics and without probiotics. Our results revealed that Bacteroidetes, Firmicutes, TM7, Proteobacteria, and Euryarchaeota dominate the bacterial communities. In our observations, and supplements reduced the archaeal population of Methanomassiliicocca in the T1, T2 and T3 groups, and caused an increase in the T4 group. Chlamydiae were present only in the T5 group, suggesting that probiotic and antibiotic inhibit the growth of pathogens in the rumen. We inferred, based on our results, that and favour the survival of beneficial microbial communities in the goats' rumen. This may lead to an overall improved feed efficacy and growth rate.
利用Illumina Miseq平台，针对从采集的瘤胃液中提取的16S rRNA微生物基因的V3-V4区域，研究了直接投喂益生菌单独及组合作为波尔山羊和斑点山羊饲料补充剂时对瘤胃微生物群落的影响。将36只波尔山羊和斑点山羊分为五个实验组：（T1）=日粮+；（T2）=日粮+；（T3）=日粮++；（T4，阳性对照）=日粮+抗生素，（T5，阴性对照）=无抗生素和无益生菌的日粮。我们的结果显示，拟杆菌门、厚壁菌门、TM7、变形菌门和广古菌门在细菌群落中占主导地位。在我们的观察中，和补充剂降低了T1、T2和T3组中甲烷微球菌的古菌数量，并使T4组增加。衣原体仅存在于T5组，表明益生菌和抗生素抑制了瘤胃中病原体的生长。基于我们的结果，我们推断和有利于山羊瘤胃中有益微生物群落的存活。这可能会导致整体饲料效率和生长速度的提高。
