State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Molecules. 2021 Jun 10;26(12):3549. doi: 10.3390/molecules26123549.
A simple, rapid, accurate, and selective quantitative method based on H nuclear magnetic resonance (qNMR) was successfully established and developed for assessing the purity of dipotassium glycyrrhizinate (KG). In this study, using potassium hydrogen phthalate and fumaric acid as internal standard (IS), several important experimental parameters, such as relaxation delay and pulse angle, were explored. Reliability, specificity, linearity, limit of quantification, precision, stability, and accuracy were also validated. Calibration results obtained from qNMR were consistent with those obtained from HPLC coupled with ultraviolet detection. The proposed method, independent of the reference standard substance, is a useful, reliable, and practical protocol for the determination of KG and glycyrrhizin analogs.
建立并成功开发了一种基于氢核磁共振（qNMR）的简单、快速、准确和选择性的定量方法，用于评估甘草酸二钾（KG）的纯度。在这项研究中，使用邻苯二甲酸钾和富马酸作为内标（IS），探讨了几个重要的实验参数，如弛豫延迟和脉冲角。还验证了可靠性、特异性、线性、定量限、精密度、稳定性和准确性。qNMR 得到的校准结果与 HPLC 结合紫外检测得到的结果一致。该方法不依赖于对照品，是一种用于测定 KG 和甘草酸类似物的有用、可靠和实用的方法。
