Nakorn Sarun Na, Dokduang Hasaya, Namwat Nisana, Klanrit Poramate, Wangwiwatsin Arporn, Promraksa Bundit, Sitthirak Sirinya, Seaban Tinnapat, Loilome Watcharin
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Cholangiocarcinoma Research Institute, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Heliyon. 2024 Dec 6;10(24):e41010. doi: 10.1016/j.heliyon.2024.e41010. eCollection 2024 Dec 30.
Coconut water is a popular drink in tropical countries and worldwide due to its delicious taste, easy consumption and nutritionally rich properties. Our study aimed to identify bioactive compounds of coconut varieties and their antioxidant as well as longevity effects in 2 different groups of coconuts. These include the bleeding coconut varieties, which are currently most available in the market, namely the Ban Phaeo and Ratchaburi coconut varieties, and the traditional coconut varieties, including Kon-jib and Sampran coconut varieties. Proton nuclear magnetic resonance (H NMR) was used to profile the metabolites in coconut water which revealed 27 metabolites including sugar, organic acids, fatty acids, flavonoids and phenolic compounds. A multivariate data analysis demonstrated an organic acid and phenolic metabolites as the antioxidant bioactive metabolites in coconut water. In addition, the coconut varieties had higher antioxidant bioactive metabolites compared to traditional coconut varieties, while traditional coconut varieties had higher sugar levels. Furthermore, the varieties containing higher antioxidants bioactive metabolites were chosen to examine the longevity effect on human dermal fibroblasts (HDFs). The results revealed that coconut water can significantly decrease cytosolic oxidation in hydrogen peroxide treated HDFs cell line and mediated longevity of fibroblast cells by modulating the expression of proteins in longevity pathway. Data from our study indicate that coconut water serves as source of antioxidants which can be mediated longevity of dermal cell. Moreover, this study provides the useful information for the coconut water production and distribution business.
椰子水因其美味的口感、易于饮用和营养丰富的特性，在热带国家乃至全球都是一种受欢迎的饮品。我们的研究旨在确定不同品种椰子中的生物活性化合物及其抗氧化和对寿命的影响，研究对象分为两组不同的椰子。其中包括目前市场上最常见的“流血”椰子品种，即班费奥椰子和叻丕府椰子品种，以及传统椰子品种，包括孔吉布椰子和三攀椰子品种。采用质子核磁共振（H NMR）对椰子水中的代谢物进行分析，共鉴定出27种代谢物，包括糖、有机酸、脂肪酸、黄酮类化合物和酚类化合物。多变量数据分析表明，有机酸和酚类代谢物是椰子水中的抗氧化生物活性代谢物。此外，“流血”椰子品种的抗氧化生物活性代谢物含量高于传统椰子品种，而传统椰子品种的糖分含量更高。此外，选择抗氧化生物活性代谢物含量较高的品种来研究其对人皮肤成纤维细胞（HDFs）的寿命影响。结果表明，椰子水可以显著降低过氧化氢处理的HDFs细胞系中的胞质氧化，并通过调节长寿途径中蛋白质的表达来介导成纤维细胞的寿命。我们的研究数据表明，椰子水是抗氧化剂的来源，可以介导皮肤细胞的寿命。此外，本研究为椰子水的生产和分销业务提供了有用信息。
