Mena-Álvarez Jesús, Rico-Romano Cristina, Gutiérrez-Ortega Carlos, Arias-Sanz Pablo, Castro-Urda Javier
Department of Endodontics, Faculty of Dentistry, Alfonso X El Sabio University, 28691 Madrid, Spain.
Department of Epidemiology, Central Defense Hospital "Gomez Ulla", 28047 Madrid, Spain.
J Clin Med. 2021 Jun 8;10(12):2536. doi: 10.3390/jcm10122536.
The aim of this paper was to assess the differences in tissue response to implantation during 15, 30 and 45 days in the subcutaneous connective tissue of Wistar rats from three biomaterials: Angelus MTA, Theracal LC, and Angelus MTA to which 25% bioglass G3 was added. Twenty-four Wistar rats were used, the materials were inserted into the rat's dorsal area in silicone tubes 5 mm long by 1.5 mm diameter. Histological reaction was assessed at 15, 30, and 45 days after implantation. They were then stained with hematoxylin eosin and evaluated by two observers. Data were analyzed using Fisher's exact test and Mann-Whitney's U test was used to determine the association between variables. Angelus MTA induced the formation of dystrophic calcifications twice as much as Theracal LC ( < 0.05). The addition of G3 did not affect the greater or lesser occurrence of calcifications ( > 0.05). Theracal LC and MTA plus G3 caused an inflammatory reaction, which was chronic at 15 days and decreased in intensity, almost disappearing after 45 days. Theracal LC, as well as Angelus MTA plus G3, were well tolerated when implanted in the subcutaneous connective tissue of rat. Histologically, no inconvenience was found for the use by direct contact of Theracal LC, and the mixture of MTA with 25% bioactive glass G3, in the tissue of Wistar rats.
本文的目的是评估三种生物材料（安杰卢斯MTA、Theracal LC以及添加了25%生物玻璃G3的安杰卢斯MTA）在Wistar大鼠皮下结缔组织中植入15天、30天和45天时组织反应的差异。使用了24只Wistar大鼠，将材料插入大鼠背部区域直径1.5毫米、长5毫米的硅胶管中。在植入后15天、30天和45天评估组织学反应。然后用苏木精伊红染色，并由两名观察者进行评估。数据采用Fisher精确检验进行分析，使用Mann-Whitney U检验确定变量之间的关联。安杰卢斯MTA诱导的营养不良性钙化形成量是Theracal LC的两倍（P < 0.05）。添加G3并未影响钙化的多少（P > 0.05）。Theracal LC和MTA加G3引发了炎症反应，在15天时为慢性炎症，强度随后降低，45天后几乎消失。当植入大鼠皮下结缔组织时，Theracal LC以及安杰卢斯MTA加G3耐受性良好。组织学上，未发现Theracal LC以及MTA与25%生物活性玻璃G3的混合物直接接触Wistar大鼠组织时使用存在不便之处。
