Malý Martin, Hajšl Martin, Bechyňská Kamila, Kučerka Ondřej, Šrámek Martin, Suttnar Jiří, Hlaváčková Alžběta, Hajšlová Jana, Kosek Vít
Department of Medicine, First Faculty of Medicine, Charles University in Prague and Military University Hospital, U Vojenské Nemocnice 1200, 169 00 Prague, Czech Republic.
Department of Food Chemistry and Analysis, University of Chemistry and Technology, Technická 3, 166 28 Prague, Czech Republic.
Metabolites. 2021 Jun 23;11(7):412. doi: 10.3390/metabo11070412.
Alterations in lipid metabolism mediated by oxidative stress play a key role in the process of atherosclerosis and superimposed thrombosis; these can lead to acute coronary syndrome (ACS) and acute ischemic stroke (AIS). Multiple studies have shown that the formation of atheromatous lesions is initiated by oxidation of low-density lipoproteins incorporated into the intima of the vessel wall. Here, we studied lipids in plasma samples from three cohorts: 61 patients with ACS (group A), 49 patients with AIS (group D), and 82 controls (group K). Untargeted lipidomics based on high-performance liquid chromatography coupled to mass spectrometry (UHPLC-HRMS) was employed to obtain comprehensive information on whether relationships exist between these patient categories based on lipid patterns. In addition, malondialdehyde (MDA) as a standard marker of oxidative stress was monitored. The most characteristic lipids in group K were fatty acyls of hydroxyfatty acids (FAHFAs). As expected, MDA concentrations were the lowest in group K. Our findings can better explain ongoing pathologies, both acute and chronic, with the potential for future diagnosis and treatment.
氧化应激介导的脂质代谢改变在动脉粥样硬化和叠加血栓形成过程中起关键作用；这些可导致急性冠状动脉综合征（ACS）和急性缺血性中风（AIS）。多项研究表明，动脉粥样硬化病变的形成始于掺入血管壁内膜的低密度脂蛋白的氧化。在此，我们研究了来自三个队列的血浆样本中的脂质：61例ACS患者（A组）、49例AIS患者（D组）和82名对照者（K组）。采用基于高效液相色谱-质谱联用（UHPLC-HRMS）的非靶向脂质组学方法，以获取基于脂质模式的这些患者类别之间是否存在关系的全面信息。此外，监测了作为氧化应激标准标志物的丙二醛（MDA）。K组中最具特征性的脂质是羟基脂肪酸的脂肪酰基（FAHFAs）。正如预期的那样，K组中的MDA浓度最低。我们的研究结果可以更好地解释急性和慢性的当前病理情况，具有未来诊断和治疗的潜力。
