Sei Clara J, Rao Mangala, Schuman Richard F, Daum Luke T, Matyas Gary R, Rikhi Nimisha, Muema Kevin, Anderson Alexander, Jobe Ousman, Kroscher Kellie A, Alving Carl R, Fischer Gerald W
Longhorn Vaccines and Diagnostics, Gaithersburg, MD 20878, USA.
U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
Vaccines (Basel). 2021 Jun 25;9(7):698. doi: 10.3390/vaccines9070698.
A universal influenza candidate vaccine that targets multiple conserved influenza virus epitopes from hemagglutinin (HA), neuraminidase (NA) and matrix (M2e) proteins was combined with the potent Army liposomal adjuvant (ALFQ) to promote induction of broad immunity to seasonal and pandemic influenza strains. The unconjugated and CRM-conjugated composite peptides formulated with ALFQ were highly immunogenic and induced both humoral and cellular immune responses in mice. Broadly reactive serum antibodies were induced across various IgG isotypes. Mice immunized with the unconjugated composite peptide developed antibody responses earlier than mice immunized with conjugated peptides, and the IgG antibodies were broadly reactive and neutralizing across Groups 1 and 2 influenza viruses. Multi-epitope unconjugated influenza composite peptides formulated with ALFQ provide a novel strategy for the development of a universal influenza vaccine. These synthetic peptide vaccines avoid the pitfalls of egg-produced influenza vaccines and production can be scaled up rapidly and economically.
一种针对血凝素（HA）、神经氨酸酶（NA）和基质（M2e）蛋白中多个保守流感病毒表位的通用流感候选疫苗，与强效的陆军脂质体佐剂（ALFQ）联合使用，以促进对季节性和大流行性流感毒株产生广泛免疫。用ALFQ配制的未缀合和CRM缀合复合肽具有高度免疫原性，并在小鼠中诱导了体液免疫和细胞免疫反应。在各种IgG同种型中均诱导出广泛反应性血清抗体。用未缀合复合肽免疫的小鼠比用缀合肽免疫的小鼠更早产生抗体反应，并且IgG抗体对第1组和第2组流感病毒具有广泛反应性和中和作用。用ALFQ配制的多表位未缀合流感复合肽为通用流感疫苗的开发提供了一种新策略。这些合成肽疫苗避免了鸡蛋生产的流感疫苗的缺陷，并且生产可以快速且经济地扩大规模。
