Chotphruethipong Lalita, Binlateh Thunwa, Hutamekalin Pilaiwanwadee, Aluko Rotimi E, Tepaamorndech Surapun, Zhang Bin, Benjakul Soottawat
International Center of Excellence in Seafood Science and Innovation, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
School of Geriatric Oral Health, Institute of Dentistry, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Foods. 2021 Jun 25;10(7):1476. doi: 10.3390/foods10071476.
Osteoporosis is a serious problem affecting health of the elderly. Drugs (bisphosphonates) applied for treatment are often accompanied by adverse side effects. Thus, fish byproduct-derived peptides, particularly hydrolyzed collagen (HC) from defatted sea bass skin, could be a safe source of anti-osteoporosis agents. This study aimed to examine the effects of HC on proliferation and differentiation of preosteoblast cells. HC prepared using papain before Alcalase hydrolysis was determined for molecular weight (MW) distribution. Thereafter, the resulting HC (50-800 µg/mL) was added to the cell. Proliferation, alkaline phosphatase activity (AP-A) and mineralization of cells were investigated. Moreover, the expression of runt-related transcription factor 2 (RUNX2) and the p-Akt/Akt pathway were also determined using Western blot. The results showed that HC had an MW < 3 kDa. HC (50-200 µg/mL) could promote cell proliferation. Nevertheless, HC at 100 µg/mL (HC-100) had enhanced AP-A and increased mineralization during the first 7 days of culture. Moreover, HC-treated cells had higher calcium depositions than the control ( < 0.05). Additionally, cells treated with HC-100 had higher levels of RUNX2 and p-Akt expressions than control ( < 0.05). Therefore, HC could be a promising functional ingredient to promote osteoblast proliferation and differentiation, which could enhance bone strength.
骨质疏松症是一个影响老年人健康的严重问题。用于治疗的药物（双膦酸盐）常常伴有不良副作用。因此，鱼副产品衍生的肽，特别是来自脱脂海鲈皮的水解胶原蛋白（HC），可能是抗骨质疏松药物的安全来源。本研究旨在考察HC对成骨前体细胞增殖和分化的影响。测定了在碱性蛋白酶水解之前用木瓜蛋白酶制备的HC的分子量（MW）分布。此后，将所得的HC（50 - 800μg/mL）添加到细胞中。研究了细胞的增殖、碱性磷酸酶活性（AP-A）和矿化情况。此外，还使用蛋白质印迹法测定了 runt 相关转录因子 2（RUNX2）的表达以及 p-Akt/Akt 信号通路。结果表明，HC 的分子量<3 kDa。HC（50 - 200μg/mL）可促进细胞增殖。然而，在培养的前 7 天，100μg/mL 的 HC（HC-100）具有增强的 AP-A 并增加了矿化。此外，HC 处理的细胞比对照组具有更高的钙沉积（<0.05）。另外，用 HC-100 处理的细胞比对照组具有更高水平的 RUNX2 和 p-Akt 表达（<0.05）。因此，HC 可能是一种有前景的功能性成分，可促进成骨细胞增殖和分化，从而增强骨强度。
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