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来自太平洋白对虾壳聚糖的壳寡糖通过NF-κB、Erk1/2、Akt和Nrf2/HO-1信号通路改善脂多糖诱导的RAW264.7巨噬细胞中的炎症和氧化应激。

Chitooligosaccharide from Pacific White Shrimp Shell Chitosan Ameliorates Inflammation and Oxidative Stress via NF-κB, Erk1/2, Akt and Nrf2/HO-1 Pathways in LPS-Induced RAW264.7 Macrophage Cells.

作者信息

Chotphruethipong Lalita, Chanvorachote Pithi, Reudhabibadh Ratchaneekorn, Singh Avtar, Benjakul Soottawat, Roytrakul Sittiruk, Hutamekalin Pilaiwanwadee

机构信息

Department of Food Science, Faculty of Science, Burapha University, Mueang Chonburi, Chonburi 20131, Thailand.

Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.

出版信息

Foods. 2023 Jul 19;12(14):2740. doi: 10.3390/foods12142740.

DOI:10.3390/foods12142740
PMID:37509832
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10379839/
Abstract

Chitooligosaccharide (COS), found in both insects and marine sources, has several bioactivities, such as anti-inflammation and antioxidant activities. However, the mechanism of shrimp shell COS on retardation of inflammatory and antioxidant effects is limited. Therefore, the aim of this study is to examine the mechanism of the aforementioned activities of COS in LPS-activated RAW264.7 macrophage cells. COS significantly improved cell viability in LPS-activated cells. COS at the level of 500 µg/mL could reduce the TNF-α, NO and IL-6 generations in LPS-activated cells ( < 0.05). Furthermore, COS could reduce ROS formation, NF-κB overactivation, phosphorylation of Erk1/2 and Akt and Nrf2/HO-1 in LPS-exposed cells. These results indicate that COS manifests anti-inflammatory activity and antioxidant action via NF-κB, Erk1/2, Akt and Nrf2/HO-1 signaling with an increasing relevance for inflammatory disorders.

摘要

壳寡糖（COS）存在于昆虫和海洋来源中，具有多种生物活性，如抗炎和抗氧化活性。然而，虾壳COS延缓炎症和抗氧化作用的机制尚有限。因此，本研究的目的是探讨COS在脂多糖（LPS）激活的RAW264.7巨噬细胞中上述活性的机制。COS显著提高了LPS激活细胞的活力。500μg/mL水平的COS可降低LPS激活细胞中肿瘤坏死因子-α（TNF-α）、一氧化氮（NO）和白细胞介素-6（IL-6）的生成（P<0.05）。此外，COS可减少LPS暴露细胞中活性氧（ROS）的形成、核因子-κB（NF-κB）的过度激活、细胞外信号调节激酶1/2（Erk1/2）和蛋白激酶B（Akt）的磷酸化以及核因子E2相关因子2/血红素加氧酶-1（Nrf2/HO-1）。这些结果表明，COS通过NF-κB、Erk1/2、Akt和Nrf2/HO-1信号通路表现出抗炎活性和抗氧化作用，且与炎症性疾病的相关性日益增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/639f7434bd93/foods-12-02740-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/7f813dd22509/foods-12-02740-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/f7c2df004740/foods-12-02740-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/e1f166a7b8ef/foods-12-02740-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/a3e226250125/foods-12-02740-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/d017f953a1a8/foods-12-02740-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/0dcb5c8412ee/foods-12-02740-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/639f7434bd93/foods-12-02740-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/7f813dd22509/foods-12-02740-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/f7c2df004740/foods-12-02740-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/e1f166a7b8ef/foods-12-02740-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/a3e226250125/foods-12-02740-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/d017f953a1a8/foods-12-02740-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/0dcb5c8412ee/foods-12-02740-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/06e6/10379839/639f7434bd93/foods-12-02740-g007.jpg

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本文引用的文献

[1]
The influence of L-proline and fulvic acid on oxidative stress and semen quality of buffalo bull semen following cryopreservation.

Vet Med Sci. 2023-7

[2]
Chitooligosaccharides Derivatives Protect ARPE-19 Cells against Acrolein-Induced Oxidative Injury.

Mar Drugs. 2023-2-22

[3]
Attenuation of Lipopolysaccharide-Induced Inflammatory Responses through Inhibition of the NF-κB Pathway and the Increased NRF2 Level by a Flavonol-Enriched -Butanol Fraction from .

ACS Omega. 2023-1-31

[4]
Anti-Inflammatory, Antioxidative, and Nitric Oxide-Scavenging Activities of a Quercetin Nanosuspension with Polyethylene Glycol in LPS-Induced RAW 264.7 Macrophages.

Molecules. 2022-11-1

[5]
Chitooligosaccharide prevents vascular endothelial cell apoptosis by attenuation of endoplasmic reticulum stress via suppression of oxidative stress through Nrf2-SOD1 up-regulation.

Pharm Biol. 2022-12

[6]
Potential Medical Applications of Chitooligosaccharides.

Polymers (Basel). 2022-8-29

[7]
Activation of Nrf2 by Esculetin Mitigates Inflammatory Responses through Suppression of NF-κB Signaling Cascade in RAW 264.7 Cells.

Molecules. 2022-8-12

[8]
Conjugate between hydrolyzed collagen from defatted seabass skin and epigallocatechin gallate (EGCG): characteristics, antioxidant activity and cellular bioactivity.

RSC Adv. 2021-1-8

[9]
Chitosan Oligosaccharides Alleviate Colitis by Regulating Intestinal Microbiota and PPARγ/SIRT1-Mediated NF-κB Pathway.

Mar Drugs. 2022-1-24

[10]
Plantanone C attenuates LPS-stimulated inflammation by inhibiting NF-κB/iNOS/COX-2/MAPKs/Akt pathways in RAW 264.7 macrophages.

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