Chotphruethipong Lalita, Sukketsiri Wanida, Aluko Rotimi E, Sae-Leaw Thanasak, Benjakul Soottawat
Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90110 Thailand.
Department of Pharmacology, Faculty of Science, Prince of Songkla University, 15, Hat Yai, Songkhla 90110 Thailand.
J Food Sci Technol. 2021 Feb;58(2):541-551. doi: 10.1007/s13197-020-04566-4. Epub 2020 Jun 5.
Hydrolyzed collagen from the defatted Asian sea bass () (Asbs-HC) had high hydrophobic amino acids and imino acids. When fibroblast cell was treated with Asbs-HC, there was no cytotoxicity at any concentrations (25-1000 µg/mL). Asbs-HC at 1000 µg/mL exhibited the highest cell proliferation and cell migration ( < 0.05), indicating wound healing ability. Antioxidative activities of Asbs-HC at different concentrations were determined. ABTS radical scavenging activity (ABTS-RSA) and oxygen radical absorbance capacity (ORAC) increased when Asbs-HC levels augmented up to 1 mg/mL ( < 0.05). Decreased activities in scavenging DPPH radical and chelating metal were found at higher levels of Asbs-HC (0.5 and 1 mg/mL) ( < 0.05). Molecular weight (MW) of peptides in Asbs-HC ranged from 406 to 16,120 Da. Peptide containing MW of 406 Da rendered the highest scavenging activity towards ABTS radical. Thus, Asbs-HC could be applied as antioxidant, skin nourishment and wound healing agents for food/drink fortification.
来自脱脂尖吻鲈（）的水解胶原蛋白（Asbs-HC）含有高含量的疏水氨基酸和亚氨基酸。用Asbs-HC处理成纤维细胞时，在任何浓度（25 - 1000μg/mL）下均无细胞毒性。1000μg/mL的Asbs-HC表现出最高的细胞增殖和细胞迁移能力（<0.05），表明其具有伤口愈合能力。测定了不同浓度Asbs-HC的抗氧化活性。当Asbs-HC水平增加至1mg/mL时，ABTS自由基清除活性（ABTS-RSA）和氧自由基吸收能力（ORAC）增强（<0.05）。在较高水平的Asbs-HC（0.5和1mg/mL）下，发现清除DPPH自由基和螯合金属的活性降低（<0.05）。Asbs-HC中肽的分子量（MW）范围为406至16,120Da。分子量为406Da的肽对ABTS自由基的清除活性最高。因此，Asbs-HC可作为抗氧化剂、皮肤营养剂和伤口愈合剂用于食品/饮料强化。
