Shyam Chitralekha, Tripathi Manoj Kumar, Tiwari Sushma, Tripathi Niraj, Solanki Ravindra Singh, Sapre Swapnil, Ahuja Ashok, Tiwari Sharad
Department of Genetics & Plant Breeding, College of Agriculture, RVS Agriculture University, Gwalior 474002, India.
Department of Plant Molecular Biology & Biotechnology, College of Agriculture, RVS Agriculture University, Gwalior 474002, India.
Plants (Basel). 2021 Jun 25;10(7):1297. doi: 10.3390/plants10071297.
is a crucial cultivated mustard species and principal oilseed crop of India and Madhya Pradesh, grown for diverse vegetables, condiments, and oilseeds. Somaclonal variation was explored as a probable source of additional variability for the manipulation of fatty acids, especially low erucic acid contents that may be valuable for this commercially important plant species. The plantlets regenerated from tissue cultures (R), their R generation and respective parental lines were compared for morpho-physiological traits and fatty acid profile for the probable existence of somaclonal variations. The first putative somaclone derived from genotype CS54 contained 5.48% and 5.52% erucic acid in R and R regenerants, respectively, compared to the mother plant (41.36%). In comparison, the second somaclone acquired from PM30 exhibited a complete absence of erucic acid corresponding to its mother plant (1.07%). These putative somaclones present a source of variation for exploitation in the development of future mustard crops with low erucic acid content.
是印度和中央邦一种至关重要的栽培芥菜品种及主要油料作物，用于种植多种蔬菜、调味品和油料种子。体细胞克隆变异被视作一种可能的额外变异来源，用于调控脂肪酸，尤其是低芥酸含量，这对这种具有重要商业价值的植物物种可能具有重要意义。比较了从组织培养再生的植株（R）、其R代及各自亲本品系的形态生理性状和脂肪酸谱，以探寻体细胞克隆变异的可能存在情况。源自基因型CS54的首个推定体细胞克隆在R代和R再生植株中的芥酸含量分别为5.48%和5.52%，而母本植株的芥酸含量为41.36%。相比之下，从PM30获得的第二个体细胞克隆的芥酸含量与其母本植株（1.07%）相比完全没有芥酸。这些推定体细胞克隆为未来培育低芥酸含量芥菜作物提供了变异来源以供利用。
