• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于检测甘露聚糖的EBCA-1单克隆抗体碳水化合物特异性的重新研究。

Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Mannan.

作者信息

Krylov Vadim B, Solovev Arsenii S, Puchkin Ilya A, Yashunsky Dmitry V, Antonets Anna V, Kutsevalova Olga Y, Nifantiev Nikolay E

机构信息

Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciencesa, Leninsky Prospect 47, 119991 Moscow, Russia.

Medical Genetic Center, Rostov-on-Don State Medical University, Nakhichevansky, 29, 344022 Rostov-on-Don, Russia.

出版信息

J Fungi (Basel). 2021 Jun 24;7(7):504. doi: 10.3390/jof7070504.

DOI:10.3390/jof7070504
PMID:34202579
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8303853/
Abstract

Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to distinct fragments of mannan were loaded onto a streptavidin-coated plate to form a glycoarray. Its use demonstrated that EBCA-1 recognizes the trisaccharide β-Man-(1→2)-α-Man-(1→2)-α-Man and not homo-α-(1→2)-linked pentamannoside, as was reported previously.

摘要

单克隆抗体EBCA-1用于夹心免疫测定，以检测血清样本中的循环甘露聚糖，用于侵袭性念珠菌病的诊断。为了重新研究EBCA-1的碳水化合物特异性，将一组与甘露聚糖不同片段结构相关的生物素化寡糖加载到链霉亲和素包被的板上以形成糖阵列。其应用表明，EBCA-1识别三糖β-Man-(1→2)-α-Man-(1→2)-α-Man，而不像先前报道的那样识别同型α-(1→2)连接的五甘露糖苷。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49ac/8303853/553864715e96/jof-07-00504-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49ac/8303853/88ce4c37c48b/jof-07-00504-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49ac/8303853/553864715e96/jof-07-00504-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49ac/8303853/88ce4c37c48b/jof-07-00504-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/49ac/8303853/553864715e96/jof-07-00504-g002.jpg

相似文献

1
Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Mannan.用于检测甘露聚糖的EBCA-1单克隆抗体碳水化合物特异性的重新研究。
J Fungi (Basel). 2021 Jun 24;7(7):504. doi: 10.3390/jof7070504.
2
Reinvestigation of carbohydrate specificity of EB-A2 monoclonal antibody used in the immune detection of galactomannan.用于半乳甘露聚糖免疫检测的EB - A2单克隆抗体的碳水化合物特异性再研究。
Heliyon. 2019 Jan 31;5(1):e01173. doi: 10.1016/j.heliyon.2019.e01173. eCollection 2019 Jan.
3
[Demonstration of β-1,2 mannan structures expressed on the cell wall of Candida albicans yeast form but not on the hyphal form by using monoclonal antibodies].[利用单克隆抗体证明白色念珠菌酵母形式而非菌丝形式的细胞壁上表达的β-1,2甘露聚糖结构]
Mikrobiyol Bul. 2015 Jan;49(1):66-76. doi: 10.5578/mb.8640.
4
Latex agglutination tests for detection of Candida antigens in sera of patients with invasive candidiasis.用于检测侵袭性念珠菌病患者血清中念珠菌抗原的乳胶凝集试验。
J Infect Dis. 1986 Mar;153(3):579-85. doi: 10.1093/infdis/153.3.579.
5
Identification of a new DC-SIGN binding pentamannoside epitope within the complex structure of mannan.在甘露聚糖的复杂结构中鉴定一种新的DC-SIGN结合五甘露糖苷表位。
Cell Surf. 2023 Jul 22;10:100109. doi: 10.1016/j.tcsw.2023.100109. eCollection 2023 Dec 15.
6
Comparative evaluation of (1, 3)-beta-D-glucan, mannan and anti-mannan antibodies, and Candida species-specific snPCR in patients with candidemia.念珠菌血症患者中（1, 3）-β-D-葡聚糖、甘露聚糖及抗甘露聚糖抗体与念珠菌属特异性巢式聚合酶链反应的比较评估
BMC Infect Dis. 2007 Sep 4;7:103. doi: 10.1186/1471-2334-7-103.
7
Nature of Candida albicans-derived carbohydrate antigen recognized by a monoclonal antibody in patient sera and distribution over Candida species.患者血清中一种单克隆抗体识别的白色念珠菌衍生碳水化合物抗原的性质及其在念珠菌属中的分布。
FEMS Microbiol Lett. 1998 Dec 1;169(1):131-8. doi: 10.1111/j.1574-6968.1998.tb13309.x.
8
Contribution of Candida biomarkers and DNA detection for the diagnosis of invasive candidiasis in ICU patients with severe abdominal conditions.念珠菌生物标志物和DNA检测对重症腹部疾病ICU患者侵袭性念珠菌病诊断的贡献。
Crit Care. 2016 May 16;20(1):149. doi: 10.1186/s13054-016-1324-3.
9
Cross-reactivity between the mannan of Candida species, Klebsiella K24 polysaccharide and Salmonella C1 and E O-antigens is mediated by a terminal non-reducing beta-mannosyl residue.念珠菌属的甘露聚糖、肺炎克雷伯菌K24多糖以及沙门氏菌C1和O抗原之间的交叉反应性是由一个末端非还原性β-甘露糖基残基介导的。
Eur J Biochem. 1994 Mar 15;220(3):973-9. doi: 10.1111/j.1432-1033.1994.tb18701.x.
10
Existence of novel branched side chains containing beta-1,2 and alpha-1,6 linkages corresponding to antigenic factor 9 in the mannan of Candida guilliermondii.季也蒙念珠菌甘露聚糖中存在与抗原因子9相对应的含有β-1,2和α-1,6连接的新型分支侧链。
J Biol Chem. 1996 Apr 19;271(16):9259-66. doi: 10.1074/jbc.271.16.9259.

引用本文的文献

1
ASCA-related antibodies in the blood sera of healthy donors and patients with colorectal cancer: characterization with oligosaccharides related to mannan.健康供体和结直肠癌患者血清中的抗酿酒酵母抗体（ASCA）：与甘露聚糖相关的寡糖表征
Front Mol Biosci. 2023 Dec 7;10:1296828. doi: 10.3389/fmolb.2023.1296828. eCollection 2023.
2
Synthesis of biotinylated pentasaccharide structurally related to a fragment of glucomannan from .与来自……的葡甘露聚糖片段结构相关的生物素化五糖的合成
Russ Chem Bull. 2021;70(11):2208-2213. doi: 10.1007/s11172-021-3334-9. Epub 2022 Jan 15.

本文引用的文献

1
A synthetic glycan array containing glucuronoxylomannan capsular polysaccharide fragments allows the mapping of protective epitopes.一种包含葡糖醛酸木甘露聚糖荚膜多糖片段的合成聚糖阵列可用于绘制保护性表位图谱。
Chem Sci. 2020 Aug 7;11(34):9209-9217. doi: 10.1039/d0sc01249a.
2
Dissection of the anti-Candida albicans mannan immune response using synthetic oligomannosides reveals unique properties of β-1,2 mannotriose protective epitopes.利用合成寡甘露糖揭示抗白念珠菌甘露聚糖免疫反应的独特性质β-1,2 曼三糖保护表位。
Sci Rep. 2021 May 24;11(1):10825. doi: 10.1038/s41598-021-90402-4.
3
Mechanisms of Candida Resistance to Antimycotics and Promising Ways to Overcome It: The Role of Probiotics.
念珠菌对抗真菌药物耐药的机制及克服耐药的有前途的方法：益生菌的作用。
Probiotics Antimicrob Proteins. 2021 Aug;13(4):926-948. doi: 10.1007/s12602-021-09776-6. Epub 2021 Mar 18.
4
Synthetic carbohydrate based anti-fungal vaccines.基于合成碳水化合物的抗真菌疫苗。
Drug Discov Today Technol. 2020 Dec;35-36:35-43. doi: 10.1016/j.ddtec.2020.11.002. Epub 2020 Dec 7.
5
Potential of Chemically Synthesized Oligosaccharides To Define the Carbohydrate Moieties of the Fungal Cell Wall Responsible for the Human Immune Response, Using Aspergillus fumigatus Galactomannan as a Model.利用烟曲霉半乳甘露聚糖作为模型，探讨化学合成寡糖在定义真菌细胞壁中负责人类免疫反应的碳水化合物部分方面的潜力。
mSphere. 2020 Jan 8;5(1):e00688-19. doi: 10.1128/mSphere.00688-19.
6
Biotinylated Oligo-α-(1 → 4)-d-galactosamines and Their N-Acetylated Derivatives: α-Stereoselective Synthesis and Immunology Application.生物素化寡糖-α-(1 → 4)-d-半乳糖胺及其乙酰化衍生物的 α-立体选择性合成及免疫学应用。
J Am Chem Soc. 2020 Jan 22;142(3):1175-1179. doi: 10.1021/jacs.9b11703. Epub 2020 Jan 10.
7
Synthetic Oligosaccharides Mimicking Fungal Cell Wall Polysaccharides.合成寡糖模拟真菌细胞壁多糖。
Curr Top Microbiol Immunol. 2020;425:1-16. doi: 10.1007/82_2019_187.
8
Monoclonal Antibody AP3 Binds Galactomannan Antigens Displayed by the Pathogens , and .单克隆抗体 AP3 结合由病原体 和 展示的半乳甘露聚糖抗原。
Front Cell Infect Microbiol. 2019 Jul 16;9:234. doi: 10.3389/fcimb.2019.00234. eCollection 2019.
9
Novel mouse monoclonal antibodies specifically recognizing β-(1→3)-D-glucan antigen.新型鼠源单克隆抗体特异性识别β-(1→3)-D-葡聚糖抗原。
PLoS One. 2019 Apr 25;14(4):e0215535. doi: 10.1371/journal.pone.0215535. eCollection 2019.
10
Reinvestigation of carbohydrate specificity of EB-A2 monoclonal antibody used in the immune detection of galactomannan.用于半乳甘露聚糖免疫检测的EB - A2单克隆抗体的碳水化合物特异性再研究。
Heliyon. 2019 Jan 31;5(1):e01173. doi: 10.1016/j.heliyon.2019.e01173. eCollection 2019 Jan.