Tárnoki-Zách Júlia, Mehes Elod, Varga-Medveczky Zsófia, Isai Dona Greta, Barany Nandor, Bugyik Edina, Revesz Zsolt, Paku Sándor, Erdo Franciska, Czirok Andras
Department of Biological Physics, Eotvos University, 1117 Budapest, Hungary.
Faculty of Information Technology and Bionics, Pázmány Péter Catholic University, 1083 Budapest, Hungary.
Pharmaceutics. 2021 Jun 20;13(6):910. doi: 10.3390/pharmaceutics13060910.
There is an increasing demand for transdermal transport measurements to optimize topical drug formulations and to achieve proper penetration profile of cosmetic ingredients. Reflecting ethical concerns the use of both human and animal tissues is becoming more restricted. Therefore, the focus of dermal research is shifting towards in vitro assays. In the current proof-of-concept study a three-layer skin equivalent using human HaCaT keratinocytes, an electrospun polycaprolactone mesh and a collagen-I gel was compared to human excised skin samples. We measured the permeability of the samples for 2% caffeine cream using a miniaturized dynamic diffusion cell ("skin-on-a-chip" microfluidic device). Caffeine delivery exhibits similar transport kinetics through the artificial skin and the human tissue: after a rapid rise, a long-lasting high concentration steady state develops. This is markedly distinct from the kinetics measured when using cell-free constructs, where a shorter release was observable. These results imply that both the established skin equivalent and the microfluidic diffusion chamber can serve as a suitable base for further development of more complex tissue substitutes.
为了优化局部用药物制剂并实现化妆品成分的适当渗透情况，对经皮转运测量的需求日益增加。出于伦理方面的考虑，人体和动物组织的使用受到越来越多的限制。因此，皮肤研究的重点正转向体外试验。在当前的概念验证研究中，将使用人HaCaT角质形成细胞、电纺聚己内酯网和I型胶原凝胶构建的三层皮肤等效物与人体切除的皮肤样本进行了比较。我们使用小型化动态扩散池（“芯片上的皮肤”微流控装置）测量了样品对2%咖啡因乳膏的渗透性。咖啡因通过人造皮肤和人体组织的递送表现出相似的转运动力学：在快速上升之后，会形成持久的高浓度稳态。这与使用无细胞构建体时测得的动力学明显不同，在无细胞构建体中观察到的释放时间较短。这些结果表明，既定的皮肤等效物和微流控扩散室都可作为进一步开发更复杂组织替代物的合适基础。
