G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Pr. 100 Let Vladivostoku, 690022 Vladivostok, Russia.
School of Biomedicine, Far Eastern Federal University, 8 Sukhanova St., 690090 Vladivostok, Russia.
Molecules. 2021 Jun 28;26(13):3956. doi: 10.3390/molecules26133956.
Here, we investigated general porin regulation in 488, the causative agent of Far Eastern scarlet-like fever, in response to sublethal concentrations of antibiotics. We chose four antibiotics of different classes and measured gene expression using qRT-PCR and GFP reporter systems. Our data showed temporal regulation of the general porin genes and caused by antibiotic stress. The porin transcription initially decreased, providing early defensive response of the bacterium, while it returned to that of the untreated cells on prolonged antibiotic exposure. Unlike the major porin genes, the transcription of the alternative porin genes and was increased. Moreover, a short-term - and -mediated porin regulation was observed. The main finding was a phenotypic heterogeneity of population manifested in variable porin gene expression under carbenicillin exposure. This may offer adaptive fitness advantages for a particular bacterial subpopulation.
在这里，我们研究了 488 号菌（远东猩红热的病原体）在亚致死浓度抗生素作用下的一般孔蛋白调控。我们选择了四种不同类别的抗生素，并使用 qRT-PCR 和 GFP 报告系统测量基因表达。我们的数据显示，抗生素应激会导致一般孔蛋白基因 和 的时间调控。孔蛋白转录最初减少，为细菌提供了早期防御反应，而在长时间暴露于抗生素时，它又恢复到未处理细胞的水平。与主要孔蛋白基因不同，替代孔蛋白基因 和 的转录增加了。此外，还观察到一个短期的 和 介导的孔蛋白调控。主要发现是群体表型异质性，在氨苄青霉素暴露下表现为可变的孔蛋白基因表达。这可能为特定细菌亚群提供适应的优势。
