The State Key Laboratory of Freshwater Ecology and Biotechnology, The Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Int J Mol Sci. 2021 Jun 15;22(12):6414. doi: 10.3390/ijms22126414.
Natural resistance-associated macrophage proteins (Nramps) are specific metal transporters in plants with different functions among various species. The evolutionary and functional information of the gene family in has not been previously reported in detail. To identify the genes in , we performed genome-wide identification, characterization, classification, and cis-elements analysis among 22 species with 138 amino acid sequences. We also conducted chromosomal localization and analyzed the synteny relationship, promoter, subcellular localization, and expression patterns in . β-Glucuronidase staining indicated that and mainly accumulated in the root and joint between mother and daughter frond. Moreover, was also widely displayed in the frond. was intensively distributed in the root and frond. Quantitative real-time PCR results proved that the gene expression level was influenced by Cd stress, especially in response to Fe or Mn deficiency. The study provides detailed information on the gene family and their distribution and expression, laying a beneficial foundation for functional research.
天然抗性相关巨噬细胞蛋白（Nramps）是植物中具有不同功能的特定金属转运蛋白。在 中，该基因家族的进化和功能信息以前没有详细报道过。为了鉴定 中的 基因，我们在 22 个物种中进行了全基因组鉴定、特征描述、分类和顺式元件分析，共获得了 138 个氨基酸序列。我们还进行了染色体定位，并分析了 在 中的基因座、启动子、亚细胞定位和表达模式。β-葡萄糖醛酸酶染色表明 和 主要在根和母叶与子叶之间的连接处积累。此外， 在叶中也广泛表达。 在根和叶中分布广泛。实时定量 PCR 结果表明， 基因的表达水平受到 Cd 胁迫的影响，特别是对 Fe 或 Mn 缺乏的反应。该研究提供了关于 基因家族及其分布和表达的详细信息，为功能研究奠定了有益的基础。
Zotero 插件
