Wu S J, Li S E
Faculty of Basic Medicine, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing.
Sci Sin B. 1988 May;31(5):590-5.
With cDNA fragments of CPS1, OCT and ACT as probes, dot and Northern blot analyses of poly(A)+-RNA from rat liver with different pathological lesions during carcinogenesis induced by diethylnitrosamine were conducted. It was shown that the level of mRNA of tissue-specific enzymes, CPSI and OCT decreased while that of the proliferating enzyme ACT mRNA increased, and the alteration was correlated with the degree of pathological changes in each case. The relative changes in the mRNA level of these enzymes during hepatocarcinogenesis were correlated with that of enzyme activities. Implication of these findings in the mechanism of carcinogenesis in terms of cell proliferation and differentiation was discussed.
以CPS1、OCT和ACT的cDNA片段为探针，对二乙基亚硝胺诱导致癌过程中大鼠肝脏不同病理损伤的poly(A)+ -RNA进行斑点杂交和Northern印迹分析。结果表明，组织特异性酶CPSI和OCT的mRNA水平降低，而增殖酶ACT的mRNA水平升高，且这种变化与每种情况下的病理变化程度相关。这些酶在肝癌发生过程中mRNA水平的相对变化与酶活性的变化相关。讨论了这些发现对细胞增殖和分化致癌机制的意义。
