编码氨甲酰磷酸合成酶I的cDNA克隆及肝癌发生过程中CPS1 mRNA水平的变化。
Cloning of cDNA coding for carbamyl phosphate synthetase I and changes in levels of CPS1 mRNA during hepatocarcinogenesis.
作者信息
Wu S J, Li S E, Zhang H L, Luo J, Yu Y
机构信息
Faculty of Basic Medical Sciences, Peking Union Medical College, Beijing.
出版信息
Sci Sin B. 1988 Feb;31(2):197-203.
cDNA coding for carbamyl phosphate synthetase I was cloned from recombinant plasmid with insert complementary to the mRNA for CPS1 followed by hybrid-selected translation screening. The length of the insert CPS1 cDNA was approximately 800 base pairs. Using this cDNA as a probe, it was found by dot-blot analysis of the total RNAs and poly(A)+-RNAs isolated from rat livers with different pathological lesions induced by diethylnitrosamine that the levels of CPS1 mRNA were decreased, the decrease being correlated with the malignancy of hepatocytes during carcinogenesis.
编码氨甲酰磷酸合成酶I的互补DNA（cDNA）是从重组质粒中克隆出来的，该重组质粒带有与氨甲酰磷酸合成酶1（CPS1）的信使核糖核酸（mRNA）互补的插入片段，随后经过杂交选择翻译筛选。插入的CPS1 cDNA长度约为800个碱基对。以该cDNA为探针，通过对从经二乙基亚硝胺诱导产生不同病理损伤的大鼠肝脏中分离出的总核糖核酸（RNAs）和聚腺苷酸加尾核糖核酸（poly(A)+-RNAs）进行斑点印迹分析发现，CPS1信使核糖核酸的水平降低，这种降低与致癌过程中肝细胞的恶性程度相关。
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