Ponce-Rodríguez Henry Daniel, Verdú-Andrés Jorge, Campíns-Falcó Pilar, Herráez-Hernández Rosa
MINTOTA Research Group, Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100 Burjassot, València, Spain.
Departamento de Control Químico, Facultad de Química y Farmacia, Universidad Nacional Autónoma de Honduras, Ciudad Universitaria, Tegucigalpa 11101, Honduras.
Pharmaceuticals (Basel). 2021 Jun 17;14(6):580. doi: 10.3390/ph14060580.
Dietary supplements of botanical origin are increasingly consumed due to their content of plant constituents with potential benefits on health and wellness. Among those constituents, terpenes are gaining attention because of their diverse biological activities (anti-inflammatory, antibacterial, geroprotective, and others). While most of the existing analytical methods have focused on establishing the terpenic fingerprint of some plants, typically by gas chromatography, methods capable of quantifying representative terpenes in herbal preparations and dietary supplements with combined high sensitivity and precision, simplicity, and high throughput are still necessary. In this study, we have explored the utility of capillary liquid chromatography (CapLC) with diode array detection (DAD) for the determination of different terpenes, namely limonene, linalool, farnesene, α-pinene, and myrcene. An innovative method is proposed that can be applied to quantify the targets at concentration levels as low as 0.006 mg per gram of sample with satisfactory precision, and a total analysis time <30 min per sample. The reliability of the proposed method has been tested by analyzing different dietary supplements of botanical origin, namely three green coffee extract-based products, two fat burnings containing (bitter orange), and an herbal preparation containing lime and leaves of orange trees.
由于植物来源的膳食补充剂含有对健康有益的植物成分，其消费量日益增加。在这些成分中，萜类化合物因其多样的生物活性（抗炎、抗菌、保护衰老等）而受到关注。虽然现有的大多数分析方法都集中在建立某些植物的萜类指纹图谱上，通常采用气相色谱法，但仍需要能够以高灵敏度、高精度、简单性和高通量相结合的方式对草药制剂和膳食补充剂中的代表性萜类化合物进行定量的方法。在本研究中，我们探索了采用二极管阵列检测（DAD）的毛细管液相色谱（CapLC）在测定不同萜类化合物（即柠檬烯、芳樟醇、法呢烯、α-蒎烯和月桂烯）方面的效用。提出了一种创新方法，该方法可用于在每克样品低至0.006毫克的浓度水平下对目标物进行定量，精密度令人满意，每个样品的总分析时间<30分钟。通过分析不同植物来源的膳食补充剂，即三种基于绿咖啡提取物的产品、两种含有苦橙的脂肪燃烧剂以及一种含有酸橙和橙树叶的草药制剂，对所提出方法的可靠性进行了测试。
