Xia He, Li Ang, Man Jia, Li Jianyong, Li Jianfeng
Key Laboratory of High Efficiency and Clean Mechanical Manufacture of MOE, School of Mechanical Engineering, Shandong University, Jinan 250061, China.
Key National Demonstration Center for Experimental Mechanical Engineering Education, Shandong University, Jinan 250061, China.
Micromachines (Basel). 2021 Jun 19;12(6):723. doi: 10.3390/mi12060723.
In this work, we used a co-flow microfluidic device with an injection and a collection tube to generate droplets with different layers due to phase separation. The phase separation system consisted of poly(ethylene glycol) diacrylate 700 (PEGDA 700), PEGDA 250, and sodium alginate aqueous solution. When the mixture droplets formed in the outer phase, PEGDA 700 in the droplets would transfer into the outer aqueous solution, while PEGDA 250 still stayed in the initial droplet, breaking the miscibility equilibrium of the mixture and triggering the phase separation. As the phase separation proceeded, new cores emerged in the droplets, gradually forming the second and third layers. Emulsion droplets with different layers were polymerized under ultraviolet (UV) irradiation at different stages of phase separation to obtain microspheres. Microspheres with different layers showed various release behaviors in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF). The release rate decreased with the increase in the number of layers, which showed a potential application in sustained drug release.
在这项工作中，我们使用了一种带有注射管和收集管的共流微流控装置，通过相分离来生成具有不同层的液滴。相分离系统由聚乙二醇二丙烯酸酯700（PEGDA 700）、PEGDA 250和海藻酸钠水溶液组成。当在外相形成混合液滴时，液滴中的PEGDA 700会转移到外部水溶液中，而PEGDA 250仍留在初始液滴中，打破了混合物的混溶平衡并引发相分离。随着相分离的进行，液滴中出现新的核，逐渐形成第二层和第三层。在相分离的不同阶段，对具有不同层的乳液液滴进行紫外线（UV）照射聚合，以获得微球。不同层的微球在模拟胃液（SGF）和模拟肠液（SIF）中表现出不同的释放行为。释放速率随着层数的增加而降低，这表明其在药物缓释方面具有潜在应用。
