Department of Agricultural and Environmental Sciences, University of Milan, 20133 Milano, Italy.
Department of Health, Animal Science and Food Safety, University of Milan, 20133 Milano, Italy.
Cells. 2021 Jun 19;10(6):1555. doi: 10.3390/cells10061555.
We derived two novel cell lines from rainbow trout (RT) proximal (RTpi-MI) and distal intestine (RTdi-MI) and compared them with the previously established continuous cell line RTgutGC. Intestinal stem cells, differentiating and differentiated epithelial cells, and connective cells were found in all cell lines. The cell lines formed a polarized barrier, which was not permeable to large molecules and absorbed proline and glucose. High seeding density induced their differentiation into more mature phenotypes, as indicated by the downregulation of intestinal stem cell-related genes (i.e., , and ), whereas alkaline phosphatase activity was upregulated. Other enterocyte markers (i.e., sglt1 and pept1), however, were not regulated as expected. In all cell lines, the presence of a mixed population of epithelial and stromal cells was characterized for the first time. The expression by the stromal component of , a stem cell niche regulatory molecule, may explain why these lines proliferate stably in vitro. Although most parameters were conserved among the three cell lines, some significant differences were observed, suggesting that characteristics typical of each tract are partly conserved in vitro as well.
我们从虹鳟鱼的近端（RTpi-MI）和远端肠道（RTdi-MI）中衍生出两种新的细胞系，并将其与之前建立的连续细胞系 RTgutGC 进行了比较。在所有细胞系中都发现了肠道干细胞、分化和分化的上皮细胞以及连接细胞。这些细胞系形成了一个极化的屏障，对大分子是不可渗透的，并且能够吸收脯氨酸和葡萄糖。高接种密度诱导它们分化为更成熟的表型，这表现为肠道干细胞相关基因（即 、 和 ）的下调，而碱性磷酸酶活性则上调。然而，其他肠细胞标记物（即 sglt1 和 pept1）并没有按照预期进行调节。在所有细胞系中，首次描述了上皮细胞和基质细胞混合群体的存在。基质成分表达 ，一种干细胞生态位调节分子，这可能解释了为什么这些细胞系在体外能够稳定增殖。尽管这三种细胞系的大多数参数都保持一致，但也观察到了一些显著差异，这表明每个肠道区域的特征在体外也部分得到了保留。
