Department of Molecular Biology and Biotechnology, Institute of Plant Protection-National Research Institute, Władysława Węgorka 20, 60-318 Poznań, Poland.
Cells. 2021 Jun 18;10(6):1546. doi: 10.3390/cells10061546.
species, commonly known as fungus gnats, are ubiquitous in greenhouses, nurseries of horticultural plants, and commercial mushroom houses, causing significant economic losses. Moreover, the insects from the genus have a well-documented role in plant pathogenic fungi transmission. Here, a study on the potential of to acquire and transmit the peanut stunt virus (PSV) from plant to plant was undertaken. Four-day-old larvae of were exposed to PSV-P strain by feeding on virus-infected leaves of and then transferred to healthy plants in laboratory conditions. Using the reverse transcription-polymerase chain reaction (RT-PCR), real-time PCR (RT-qPCR), and digital droplet PCR (RT-ddPCR), the PSV RNAs in the larva, pupa, and imago of were detected and quantified. The presence of PSV genomic RNA strands as well as viral coat protein in , on which the viruliferous larvae were feeding, was also confirmed at the molecular level, even though the characteristic symptoms of PSV infection were not observed. The results have shown that larvae of could acquire the virus and transmit it to healthy plants. Moreover, it has been proven that PSV might persist in the insect body transstadially. Although the molecular mechanisms of virion acquisition and retention during insect development need further studies, this is the first report on playing a potential role in plant virus transmission.
种，通常被称为菌蚊，在温室、园艺植物苗圃和商业蘑菇房中无处不在，造成了巨大的经济损失。此外，该属的昆虫在植物病原菌传播方面有明确的作用。在这里，研究了 从植物到植物获取和传播花生矮化病毒（PSV）的潜力。将 4 天大的幼虫暴露在 PSV-P 株系下，通过食用受感染的花生叶片，并在实验室条件下转移到健康植物上。通过逆转录聚合酶链反应（RT-PCR）、实时 PCR（RT-qPCR）和数字液滴 PCR（RT-ddPCR），检测和定量了幼虫、蛹和成虫中 PSV 的 RNA。在 身上，以及在其上取食的带毒幼虫的 ，也在分子水平上证实了 PSV 基因组 RNA 链以及病毒外壳蛋白的存在，尽管没有观察到 PSV 感染的特征症状。结果表明，菌蚊幼虫可以获得病毒并将其传播给健康植物。此外，已经证明 PSV 可能在昆虫体内经转代而持续存在。尽管病毒粒子在昆虫发育过程中获取和保留的分子机制需要进一步研究，但这是首次报道菌蚊在植物病毒传播中可能发挥作用。
