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黄酮类化合物和酚酸含量及波兰 Wasabi 农场栽培的(Miq.)Koidz 的体外抗衰老特性研究。

Flavonoid and Phenolic Acids Content and In Vitro Study of the Potential Anti-Aging Properties of (Miq.) Koidz Cultivated in Wasabi Farm Poland.

机构信息

Department of Pharmaceutical Botany, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland.

Department of Biochemistry and Biotechnology, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland.

出版信息

Int J Mol Sci. 2021 Jun 9;22(12):6219. doi: 10.3390/ijms22126219.

Abstract

Skin aging is a natural, unavoidable, and complex process caused by oxidative stress. As a consequence, it leads to an increase in the activation of extracellular matrix disruption enzymes and DNA damage. The search for natural sources that inhibit these mechanisms can be a good approach to prevent skin aging. The purpose of our study was to evaluate the composition of flavonoids and phenolic acids in the extracts obtained from the flowers, roots, and leaves of cultivated in Poland. Then, the resultant extracts were subjected to an assessment of antioxidant, anti-collagenase, anti-elastase, anti-hyaluronidase, antibacterial, and cytotoxic properties. It was demonstrated that the extract from the flowers had the highest content of flavonoid glycosides (17.15 mg/g DE). This extract showed the greatest antioxidant, anti-collagenase, anti-elastase, and anti-hyaluronidase activities compared to the other samples. Importantly, the collagenase inhibitory activity of this extract (93.34% ± 0.77%) was better than that of positive control epigallocatechin gallate (88.49% ± 0.45%). An undeniable advantage of this extract was also to possess moderate antibacterial properties and no cytotoxicity towards normal human skin fibroblasts. Our results suggest that extracts from flowers may be considered as a promising antiaging compound for applications in cosmetic formulations.

摘要

皮肤老化是一种由氧化应激引起的自然、不可避免且复杂的过程。因此,它会导致细胞外基质破坏酶和 DNA 损伤的激活增加。寻找抑制这些机制的天然来源可能是预防皮肤老化的一种好方法。我们的研究目的是评估在波兰种植的 的花、根和叶提取物中类黄酮和酚酸的组成。然后,对所得提取物进行抗氧化、抗胶原酶、抗弹性蛋白酶、抗透明质酸酶、抗菌和细胞毒性评估。结果表明,花提取物的类黄酮糖苷含量最高(17.15 mg/g DE)。与其他样品相比,该提取物具有最强的抗氧化、抗胶原酶、抗弹性蛋白酶和抗透明质酸酶活性。重要的是,该提取物的胶原酶抑制活性(93.34%±0.77%)优于阳性对照表没食子儿茶素没食子酸酯(88.49%±0.45%)。该提取物的一个不可否认的优点是还具有适度的抗菌性能,对正常人类皮肤成纤维细胞没有细胞毒性。我们的研究结果表明, 花提取物可能被认为是一种有前途的抗衰老化合物,可应用于化妆品配方中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5d28/8229664/0cb623b05c7a/ijms-22-06219-g001.jpg

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