Bogucka-Kocka Anna, Vorobets Natalia, Chrząszcz Małgorzata, Pietrzak Wioleta, Szewczyk Katarzyna
Department of Biology and Genetics, Medical University of Lublin, 4a Chodźki Str., 20-093 Lublin, Poland.
Department of Pharmacognosy and Botany, Faculty of Pharmacy, Danylo Halytsky Lviv National Medical University, 69 Pekarska Str., Lviv 79010, Ukraine.
Antioxidants (Basel). 2019 Sep 1;8(9):363. doi: 10.3390/antiox8090363.
During inflammation, reactive oxygen species (ROS) are produced in large amounts, causing oxidative stress. Several studies confirm that plant extracts rich in phenolic compounds may inhibit ROS production. For that reason, the aim of this work is the qualitative and quantitative analysis of phenolic acids and flavonoids in the etheric (LAEN) and methanolic (LAM) extracts of the fruits of Crantz, as well as their antioxidative and cytotoxic properties. Liquid chromatography-electrospray tandem mass spectroscopy (LC-ESI-MS/MS) enabled the identification of 12 phenolic acids and nine flavonoids. Both tested extracts scavenged ROS in a concentration-dependent manner. Stronger activity was observed for the methanolic extract. The cytotoxic effect of both extracts in increasing concentrations on five types of cancer cell lines was also investigated. The cytotoxicity was estimated using trypan blue vital staining. It was found that the analyzed extracts induced the apoptosis of the cells of all the tested cell lines. In conclusion, our results present that the fruits of can be a source of valuable compounds with protective effects against oxidative damage.
在炎症过程中，活性氧（ROS）大量产生，导致氧化应激。多项研究证实，富含酚类化合物的植物提取物可能抑制ROS的产生。因此，本研究的目的是对克兰茨果实的乙醚提取物（LAEN）和甲醇提取物（LAM）中的酚酸和黄酮类化合物进行定性和定量分析，以及研究它们的抗氧化和细胞毒性特性。液相色谱-电喷雾串联质谱（LC-ESI-MS/MS）能够鉴定出12种酚酸和9种黄酮类化合物。两种受试提取物均以浓度依赖的方式清除ROS。甲醇提取物表现出更强的活性。还研究了两种提取物在浓度增加时对五种癌细胞系的细胞毒性作用。使用台盼蓝活细胞染色法评估细胞毒性。结果发现，所分析的提取物诱导了所有受试细胞系的细胞凋亡。总之，我们的结果表明，克兰茨果实可能是具有抗氧化损伤保护作用的有价值化合物的来源。
