Younis Mai M, Ayoub Iriny M, Mostafa Nada M, El Hassab Mahmoud A, Eldehna Wagdy M, Al-Rashood Sara T, Eldahshan Omayma A
Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Abbassia, Cairo 11566, Egypt.
Department of Medicinal Chemistry, Faculty of Pharmacy, King Salman International University (KSIU), South Sinai 46612, Egypt.
Plants (Basel). 2022 Mar 29;11(7):918. doi: 10.3390/plants11070918.
Today, skin care products and cosmetic preparations containing natural ingredients are widely preferred by consumers. Therefore, many cosmetic brands are encouraged to offer more natural products to the market, such as plant extracts that can be used for their antiaging, antiwrinkle, and depigmentation properties and other cosmetic purposes. In the current study, the volatile constituents of the hexane-soluble fraction of a (family Proteaceae) leaf methanol extract (SSHF) were analyzed using GC/MS analysis. Moreover, the antiaging activity of SSHF was evaluated through in vitro studies of anti-collagenase, anti-elastase, anti-tyrosinase, and anti-hyaluronidase activities. In addition, an in silico docking study was carried out to identify the interaction mechanisms of the major compounds in SSHF with the active sites of the target enzymes. Furthermore, an in silico toxicity study of the identified compounds in SSHF was performed. It was revealed that vitamin E (-tocopherol) was the major constituent of SSHF, representing 52.59% of the extract, followed by -sitosterol (8.65%), neophytadiene (8.19%), -tocopherol (6.07%), and others. The in vitro studies showed a significant inhibition by SSHF of collagenase, elastase, tyrosinase, and hyaluronidase, with IC values of 60.03, 177.5, 67.5, and 38.8 µg/mL, respectively, comparable to those of the positive controls epigallocatechin gallate (ECGC, for collagenase, elastase, hyaluronidase) and kojic acid (for tyrosinase). Additionally, the molecular docking study revealed good acceptable binding scores of the four major compounds, comparable to those of ECGC and kojic acid. Besides, the SSHF identified phytoconstituents showed no predicted potential toxicity nor skin toxicity, as determined in silico. In conclusion, the antiaging potential of SSHF may be attributed to its high content of vitamin E in addition to the synergetic effect of other volatile constituents. Thus, SSHF could be incorporated in pharmaceutical skin care products and cosmetics after further studies.
如今，含有天然成分的护肤品和化妆品制剂广受消费者青睐。因此，许多化妆品品牌受到鼓励，向市场提供更多天然产品，比如可因其抗老化、抗皱和色素沉着特性及其他美容用途而使用的植物提取物。在本研究中，采用气相色谱/质谱联用（GC/MS）分析法对一种山龙眼科植物叶片甲醇提取物（SSHF）的正己烷可溶部分的挥发性成分进行了分析。此外，通过抗胶原酶、抗弹性蛋白酶、抗酪氨酸酶和抗透明质酸酶活性的体外研究对SSHF的抗老化活性进行了评估。另外，开展了计算机对接研究，以确定SSHF中主要化合物与靶酶活性位点的相互作用机制。此外，还对SSHF中鉴定出的化合物进行了计算机毒性研究。结果显示，维生素E（α-生育酚）是SSHF的主要成分，占提取物的52.59%，其次是β-谷甾醇（8.65%）、新植二烯（8.19%）、γ-生育酚（6.07%）等。体外研究表明，SSHF对胶原酶、弹性蛋白酶、酪氨酸酶和透明质酸酶有显著抑制作用，IC值分别为60.03、177.5、67.5和38.8 μg/mL，与阳性对照表没食子儿茶素没食子酸酯（ECGC，用于胶原酶、弹性蛋白酶、透明质酸酶）和曲酸（用于酪氨酸酶）相当。此外，分子对接研究显示四种主要化合物具有良好的可接受结合分数，与ECGC和曲酸相当。此外，如计算机模拟所确定的，SSHF中鉴定出的植物成分未显示出预测的潜在毒性或皮肤毒性。总之，SSHF的抗老化潜力可能归因于其高含量的维生素E以及其他挥发性成分的协同作用。因此，经过进一步研究后，SSHF可被纳入药用护肤品和化妆品中。
