Kuchta-Gładysz Marta, Wójcik Ewa, Grzesiakowska Anna, Rymuza Katarzyna, Szeleszczuk Olga
Department of Animal Reproduction, Anatomy and Genomics, Faculty of Animal Sciences, University of Agriculture in Krakow, ul. Mickiewicza 24/28, 30-059 Kraków, Poland.
Institute of Animal Science and Fisheries, Faculty of Agrobioengineering and Animal Husbandry, Siedlce University of Natural Sciences and Humanities, ul. Prusa 14, 08-110 Siedlce, Poland.
Animals (Basel). 2021 Jun 11;11(6):1743. doi: 10.3390/ani11061743.
A cytogenetic assay based on fragile sites (FS) enables the identification of breaks, chromatid gaps, and deletions. In healthy individuals, the number of these instabilities remains low. Genome stability in these species is affected by Robertsonian translocations in the karyotype of the blue fox and by B chromosomes in the silver fox. The aims of the study were to characterise the karyotype of blue foxes, silver foxes, and their hybrids and to identify chromosomal fragile sites used to evaluate genome stability. The diploid number of A chromosomes in blue foxes ranged from 48 to 50, while the number of B chromosomes in silver foxes varied from one to four, with a constant number of A chromosomes (2n = 34). In interspecific hybrids, both types of karyotypic variation were identified, with the diploid number of A chromosomes ranging from 40 to 44 and the number of B chromosomes varying from 0 to 3. The mean frequency of FS in foxes was 4.06 ± 0.19: 4.61 ± 0.37 in blue foxes, 3.46 ± 0.28 in silver foxes, and 4.12 ± 0.22 in hybrids. A relationship was identified between an increased number of A chromosomes in the karyotype of the hybrids and the frequency of chromosomal breaks. The FS assay was used as a biomarker for the evaluation of genomic stability in the animals in the study.
基于脆性位点(FS)的细胞遗传学分析能够识别断裂、染色单体间隙和缺失。在健康个体中,这些不稳定性的数量保持在较低水平。这些物种的基因组稳定性受到蓝狐核型中的罗伯逊易位以及银狐中的B染色体的影响。本研究的目的是对蓝狐、银狐及其杂种的核型进行表征,并识别用于评估基因组稳定性的染色体脆性位点。蓝狐A染色体的二倍体数范围为48至50,而银狐B染色体的数量从1至4不等,A染色体数量恒定(2n = 34)。在种间杂种中,鉴定出了两种类型的核型变异,A染色体的二倍体数范围为40至44,B染色体的数量从0至3不等。狐狸中FS的平均频率为4.06±0.19:蓝狐为4.61±0.37,银狐为3.46±0.28,杂种为4.12±0.22。在杂种的核型中,A染色体数量的增加与染色体断裂频率之间存在关联。在本研究中,FS分析被用作评估动物基因组稳定性的生物标志物。