Institute of Animal Science and Fisheries, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland.
PLoS One. 2020 Apr 30;15(4):e0231928. doi: 10.1371/journal.pone.0231928. eCollection 2020.
Chromosome instability adversely affects animal fertility and reproduction. Analysis of instability can be a valuable diagnostic tool. Helpful tests for assessment of instabilities include the sister chromatid exchange assay, identification of fragile sites, the bleomycin assay and the comet assay. These techniques can be used to assess and compare the chromosome stability of individual breeds of animals. The aim of the study was to assess chromosome stability in boars: Duroc, Duroc x Pietrain and Pietrain x Duroc crossbreds, Polish Large White, and the Neckar, P76 and PIC lines. The study assessed the chromosome stability of boars. The distribution of instabilities in individual breeds was varied. The average frequency of chromatid exchange was 4.8 ± 1.5, while that of fragile sites was 3.9 ± 1.4. The mean level of DNA damage (% tail DNA) was 9.4 ± 8.3, while in the bleomycin assay b/c and %AM were 0.6 ± 0.7 and 44.4 ± 4.1. A higher rate of instability was found in older individuals than in younger ones. The cytogenetic assays used to identify various forms of chromosome instability can be used to evaluate boars intended for breeding.
染色体不稳定性会对动物的生育和繁殖产生不利影响。不稳定性分析可以作为一种有价值的诊断工具。有助于评估不稳定性的测试包括姐妹染色单体交换试验、脆性部位鉴定、博来霉素试验和彗星试验。这些技术可用于评估和比较动物个体品种的染色体稳定性。本研究旨在评估种猪的染色体稳定性：杜洛克猪、杜洛克猪×皮特兰猪杂交种、皮特兰猪×杜洛克猪杂交种、波兰大白猪、以及 Neckar、P76 和 PIC 系。该研究评估了种猪的染色体稳定性。不同品种的不稳定性分布各不相同。染色单体交换的平均频率为 4.8±1.5，而脆性部位的平均频率为 3.9±1.4。DNA 损伤的平均水平（%尾 DNA）为 9.4±8.3，而在博来霉素试验中 b/c 和%AM 分别为 0.6±0.7 和 44.4±4.1。与年轻个体相比，老年个体的不稳定性发生率更高。用于鉴定各种形式染色体不稳定性的细胞遗传学试验可用于评估用于繁殖的种猪。
