Petrocchi-Rilo Máximo, Martínez-Martínez Sonia, Aguarón-Turrientes Álvaro, Roca-Martínez Elisabet, García-Iglesias María-José, Pérez-Fernández Esther, González-Fernández Alba, Herencia-Lagunar Elena, Gutiérrez-Martín César-Bernardo
Departmento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, Campus de Vegazana s/n, 24071 León, Spain.
Laboratorios SYVA, Avda. de Portugal s/n, 24009 León, Spain.
Antibiotics (Basel). 2021 Jun 11;10(6):707. doi: 10.3390/antibiotics10060707.
A set of 207 isolates were collected from ten autonomous communities from Spain in 2019 to 2020 from pigs with meningitis, pneumonic lungs, arthritic joints or other swollen viscera, to a lesser extent. Thirteen capsular types were detected being the most prevalent serotype 2 (21.7%), followed by serotypes 1 (21.3%), 9 (19.3%) and 3 (6.3%). Serotypes 2 and 9 were recovered mainly from the central nervous system (CNS), while serotype 1 was isolated mostly from swollen joints and serotype 3 from the lungs. Twenty-five isolates (12.1%) could not be typed. The most prevalent pathotype was + + + (49 isolates, 23.8%), and it was related mainly to serotypes 1 and 2. Serotypes 1-3 and 9 were significantly associated with anatomical sites of isolation and virulence factors, serotype 9 (CNS) and serotypes 3 and 9 (lungs) being associated with virulence profiles without the gene. isolates showed globally high antimicrobial resistances, but ampicillin followed by spectinomycin and tiamulin resulted in the highest activities, while the greatest resistances were detected for sulphadimethoxine, tetracyclines, neomycin, clindamycin and macrolides. A total of 87.4% isolates were positive to the gene, 62.4% to the gene and 25.2% to the gene, while 14.6% were positive to all three genes simultaneously. A significative association between isolate resistances to tetracyclines and macrolides and the resistance genes tested was established, except for phenicol resistance and the gene. A set of 14 multiresistance patterns were obtained according to the number of antimicrobials to which the isolates were resistant, the resistances to 12 or more agents being the most prevalent ones. A remarkable amount of multiresistance profiles could be seen among the serotype 9 isolates.
2019年至2020年期间，从西班牙十个自治区的猪身上收集了207株分离株，这些猪患有脑膜炎、肺炎、关节炎或其他程度较轻的内脏肿胀。检测到13种荚膜类型，最常见的血清型是2型（21.7%），其次是1型（21.3%）、9型（19.3%）和3型（6.3%）。血清型2和9主要从中枢神经系统（CNS）分离得到，而血清型1大多从肿胀的关节中分离得到，血清型3则从肺部分离得到。25株分离株（12.1%）无法分型。最常见的致病型是 + + + （49株，23.8%），主要与血清型1和2相关。血清型1至3和9与分离部位及毒力因子显著相关，血清型9（CNS）以及血清型3和9（肺部）与不含 基因的毒力谱相关。分离株总体上表现出较高的抗菌耐药性，但氨苄西林其次是壮观霉素和替米考星的活性最高，而对磺胺二甲氧嘧啶、四环素、新霉素、克林霉素和大环内酯类药物的耐药性最强。共有87.4%的分离株对 基因呈阳性，62.4%对 基因呈阳性，25.2%对 基因呈阳性，而14.6%同时对这三个基因呈阳性。除了对甲砜霉素耐药性与 基因外，分离株对四环素和大环内酯类药物的耐药性与所检测的耐药基因之间建立了显著关联。根据分离株对耐药抗菌药物的数量获得了14种多重耐药模式，对12种或更多药物耐药的模式最为常见。在血清型9的分离株中可以看到大量的多重耐药谱。
