Key Laboratory of Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China.
WISH Biotechnologies, Beihu Scinece Park B, Changchun 130000, China.
Curr Issues Mol Biol. 2021 Jun 30;43(2):529-542. doi: 10.3390/cimb43020040.
Disconnected (disco)-interacting protein 2 homolog B (Dip2B) is a member of the Dip2 superfamily and plays an essential role in axonal outgrowth during embryogenesis. In adults, Dip2B is highly expressed in different brain regions, as shown by in situ analysis, and may have a role in axon guidance. However, the expression and biological role of Dip2B in other somatic tissues remain unknown. To better visualize Dip2B expression and to provide insight into the roles of Dip2B during postnatal development, we used a knock-in mouse model, in which a LacZ-Neo fusion protein is expressed under promoter and allowed Dip2B expression to be analyzed by X-gal staining. qPCR analyses showed that mRNA was expressed in a variety of somatic tissues, including lung and kidney, in addition to brain. LacZ staining indicated that Dip2B is broadly expressed in neuronal, reproductive, and vascular tissues as well as in the kidneys, heart, liver, and lungs. Moreover, neurons and epithelial cells showed rich staining. The broad and intense patterns of Dip2B expression in adult mice provide evidence of the distribution of Dip2B in multiple locations and, thereby, its implication in numerous physiological roles.
分离（disco）-相互作用蛋白 2 同源物 B（Dip2B）是 Dip2 超家族的成员，在胚胎发生过程中的轴突生长中发挥重要作用。在成年期，Dip2B 在不同的脑区中高度表达，如原位分析所示，并且可能在轴突导向中发挥作用。然而，Dip2B 在其他体组织中的表达和生物学作用仍然未知。为了更好地可视化 Dip2B 的表达，并深入了解 Dip2B 在出生后发育过程中的作用，我们使用了一个敲入小鼠模型，其中一个 LacZ-Neo 融合蛋白在 启动子的控制下表达，从而可以通过 X-gal 染色分析 Dip2B 的表达。qPCR 分析显示， 除了大脑外，在各种体组织（包括肺和肾）中也表达了 mRNA。LacZ 染色表明 Dip2B 在神经元、生殖和血管组织以及肾脏、心脏、肝脏和肺部中广泛表达。此外，神经元和上皮细胞显示出丰富的染色。Dip2B 在成年小鼠中的广泛而强烈的表达模式为 Dip2B 在多个部位的分布提供了证据，从而表明其在许多生理作用中的重要性。
