Department of Gynecology, Shandong Provincial Hospital affiliated to Shandong University, Jinan, Shandong Province, China.
Department of Gynecology, Jining NO.1 People's Hospital, Jining, Shandong Province, China.
Epigenomics. 2018 Mar;10(3):259-275. doi: 10.2217/epi-2017-0104. Epub 2018 Jan 18.
This study was intended to identify the metastasis-related miRNAs and target genes in cervical squamous cell carcinoma.
MATERIALS & METHODS: The mRNA and miRNA next-generation sequencing data were downloaded. Differential expression analysis was carried out, followed by target gene prediction of differentially expressed miRNAs. The biological function of differentially expressed genes was performed. Validation was carried out by survival analysis and qRT-PCR.
N4BP3 were associated with the survival time of patients. Hsa-mir-451 and hsa-mir-486 were related to tumor differentiation stage. Validated expression of hsa-mir-24-2, hsa-mir-582, NOTCH1, PIP4K2B, DIP2B and IGFBP5 was consistent with the bioinformatics analysis.
Alterations of miRNAs and target genes may be useful in understanding the metastasis mechanisms of cervical squamous cell carcinoma.
本研究旨在鉴定宫颈鳞癌转移相关的 miRNA 和靶基因。
下载 mRNA 和 miRNA 下一代测序数据。进行差异表达分析，然后预测差异表达 miRNA 的靶基因。对差异表达基因进行生物学功能分析。通过生存分析和 qRT-PCR 进行验证。
N4BP3 与患者的生存时间有关。hsa-mir-451 和 hsa-mir-486 与肿瘤分化阶段有关。hsa-mir-24-2、hsa-mir-582、NOTCH1、PIP4K2B、DIP2B 和 IGFBP5 的表达验证与生物信息学分析一致。
miRNA 和靶基因的改变可能有助于理解宫颈鳞癌的转移机制。
