Department of Molecular and Clinical Cancer Medicine, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Liverpool L69 3GE, UK.
Division of Diabetes endocrinology and Gastroenterology, Faculty of Biology Medicine and Health, University of Manchester, Manchester M13 9PL, UK.
Nutrients. 2021 Jun 30;13(7):2269. doi: 10.3390/nu13072269.
Altering dietary ferrous sulphate (FS) consumption exacerbates a murine model of colitis and alters the intestinal microbiome. We investigated the impact of oral ferric maltol (FM) and FS on mice with dextran sodium sulphate (DSS) induced colitis, and the microbiome of patients with iron deficiency.
Mice had acute colitis induced, with 2% DSS for 5 days, followed by water. During this period, groups of mice were fed standard chow (200 ppm iron, SC, = 8), or SC with 200ppm FS supplementation ( = 16, FSS), or SC with 200 ppm FM supplementation ( = 16, FMS). Clinical, pathological and microbiome assessments were compared at days 1 and 10. Fecal bacterial gDNA was extracted and the microbiome assessed by sequencing. Statistical inferences were made using MacQIIME. Principal Coordinates Analysis were used to visualize beta-diversity cluster analysis. Ten patients with IDA were treated with FS, and six with inactive inflammatory bowel disease received FM, supplements for four weeks: pre- and mid-treatment fecal samples were collected: the microbiome was assessed (see above).
In mice, after DSS treatment, there was a decrease in many genera in the SC and FSS groups: Lactobacillales increased in mice that received FMS. In humans, FS treatment led to an increase in five genera, but FM was not associated with any measurable change. The severity of DSS-induced colitis was greater with FSS than FMS.
This study demonstrates differential and unique influences of ferric maltol and ferrous sulphate supplements on intestinal microbiota. These differences might contribute to the different side effects associated with these preparations.
改变膳食硫酸亚铁（FS）的摄入会加剧小鼠结肠炎模型，并改变肠道微生物组。我们研究了口服麦芽酚铁（FM）和 FS 对葡聚糖硫酸钠（DSS）诱导结肠炎的小鼠以及缺铁患者微生物组的影响。
小鼠急性结肠炎模型诱导，用 2% DSS 喂养 5 天，然后用饮用水。在此期间，将小鼠分为三组：标准饮食组（200ppm 铁，SC， = 8）、标准饮食加 200ppm FS 补充剂组（FSS， = 16）和标准饮食加 200ppm FM 补充剂组（FMS， = 16）。在第 1 天和第 10 天评估临床、病理和微生物组。提取粪便细菌基因组 DNA，通过测序评估微生物组。使用 MacQIIME 进行统计推断。使用主坐标分析可视化 beta 多样性聚类分析。10 名缺铁患者接受 FS 治疗，6 名无活性炎症性肠病患者接受 FM 治疗，四周疗程：收集治疗前和中期粪便样本：如上所述评估微生物组。
在小鼠中，DSS 治疗后 SC 和 FSS 组的许多属减少：接受 FMS 的小鼠中乳杆菌属增加。在人类中，FS 治疗导致五个属增加，但 FM 与任何可测量的变化无关。与 FMS 相比，FSS 引起的 DSS 诱导结肠炎更严重。
本研究表明麦芽酚铁和硫酸亚铁补充剂对肠道微生物群有不同的、独特的影响。这些差异可能导致与这些制剂相关的不同副作用。
