Suárez Montenegro Zully J, Álvarez-Rivera Gerardo, Sánchez-Martínez José David, Gallego Rocío, Valdés Alberto, Bueno Mónica, Cifuentes Alejandro, Ibáñez Elena
Foodomics Laboratory, Institute of Food Science Research (CIAL, CSIC), Nicolas Cabrera, 9, 28049 Madrid, Spain.
Foods. 2021 Jun 29;10(7):1507. doi: 10.3390/foods10071507.
The neuroprotective potential of 32 natural extracts obtained from olive oil by-products was investigated. The online coupling of supercritical fluid extraction (SFE) and dynamic adsorption/desorption allowed the selective enrichment of olive leaves extracts in different terpenoids' families. Seven commercial adsorbents based on silica gel, zeolite, aluminum oxide, and sea sand were used with SFE at three different extraction times to evaluate their selectivity towards different terpene families. Collected fractions were analyzed by gas chromatography coupled to quadrupole-time-of-flight mass spectrometry (GC-QTOF-MS) to quantify the recoveries of monoterpenes (C10), sesquiterpenes (C15), diterpenes (C20), and triterpenes (C30). A systematic analysis of the neuroprotective activity of the natural extracts was then carried out. Thus, a set of in vitro bioactivity assays including enzymatic (acetylcholinesterase (AChE), butyrylcholinesterase (BChE)), and anti-inflammatory (lipoxidase (LOX)), as well as antioxidant (ABTS), and reactive oxygen and nitrogen species (ROS and RNS, respectively) activity tests were applied to screen for the neuroprotective potential of these extracts. Statistical analysis showed that olive leaves adsorbates from SS exhibited the highest biological activity potential in terms of neuroprotective effect. Blood-brain barrier permeation and cytotoxicity in HK-2 cells and human THP-1 monocytes were studied for the selected olive leaves fraction corroborating its potential.
研究了从橄榄油副产品中获得的32种天然提取物的神经保护潜力。超临界流体萃取（SFE）与动态吸附/解吸的在线联用使得橄榄叶提取物能够在不同萜类家族中选择性富集。使用了七种基于硅胶、沸石、氧化铝和海砂的商业吸附剂，在三个不同的萃取时间与SFE联用，以评估它们对不同萜烯家族的选择性。通过气相色谱-四极杆-飞行时间质谱联用仪（GC-QTOF-MS）对收集的馏分进行分析，以量化单萜（C10）、倍半萜（C15）、二萜（C20）和三萜（C30）的回收率。随后对天然提取物的神经保护活性进行了系统分析。因此，应用了一组体外生物活性测定方法，包括酶活性测定（乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE））、抗炎活性测定（脂氧化酶（LOX））、抗氧化活性测定（ABTS）以及活性氧和活性氮（分别为ROS和RNS）活性测试，以筛选这些提取物的神经保护潜力。统计分析表明，从海砂吸附得到的橄榄叶吸附物在神经保护作用方面表现出最高的生物活性潜力。对所选的橄榄叶馏分进行了血脑屏障通透性以及在HK-2细胞和人THP-1单核细胞中的细胞毒性研究，证实了其潜力。
