Dept. of Microbiology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India.
Dept. of Gastroenterology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India.
Indian J Med Microbiol. 2021 Oct-Dec;39(4):479-483. doi: 10.1016/j.ijmmb.2021.06.009. Epub 2021 Jun 29.
Hepatitis C virus is a major cause of chronic hepatitis with seven known genotypes. Uttarakhand is a north Indian state in the Sub-Himalayan region where the genotypic distribution of HCV is largely unknown. This study was undertaken in order to assess the pattern of genotype and subtype and understand the risk factors leading to transmission of Hepatitis C virus in this understudied region.
Anti-HCV reactive cases were selected for determination of the circulating genotypes. Viral RNA was confirmed by real-time PCR. Strains were amplified and sequenced using Sanger's methods. Phylogenetic tree was constructed to determine the genotype.
Genotype 3 was found to be the predominant genotype majority being subtype 3a and 3b followed by genotype 1. Subtypes 3g and genotype 4a were also observed. Major risk factor found was parenteral injection therapy from unregistered medical practitioners for minor ailments.
Findings of our study will help in tailoring management and prevention protocols for HCV for the people of this region.
丙型肝炎病毒是慢性肝炎的主要病因，已知有七种基因型。北印度的乌塔兰恰尔邦位于次喜马拉雅地区，其丙型肝炎病毒的基因型分布在很大程度上尚不清楚。本研究旨在评估基因型和亚型模式，并了解导致丙型肝炎病毒在这一研究不足地区传播的危险因素。
选择抗 HCV 反应性病例以确定循环基因型。通过实时 PCR 确认病毒 RNA。使用 Sanger 方法扩增和测序菌株。构建系统发育树以确定基因型。
发现基因型 3 是主要的基因型，主要亚型为 3a 和 3b，其次是基因型 1。还观察到亚型 3g 和基因型 4a。主要的危险因素是未经注册的医生为治疗小病而进行的非肠道注射治疗。
本研究的结果将有助于为该地区的人们制定丙型肝炎病毒的管理和预防方案。
