Nakamura S, Nakamura T, Kawahara H
Department of Orthopedics, Faculty of Medicine, University of Tokyo, Japan.
Acta Orthop Scand. 1988 Aug;59(4):438-40. doi: 10.3109/17453678809149398.
We studied the immunohistochemical distribution of the alpha and beta subunits of S-100 protein in human articular cartilage by the Avidin-Biotin Complex method using monoclonal antibodies against each subunit. Immunostaining for both subunits was detected in chondrocytes in superficial, intermediate, and deep zones of normal articular cartilage. In the matrix, only the superficial zone was stained positively. In arthrotic joints, we detected intense immunostaining in clustered chondrocytes in the hypercellular area, and weak or no immunostaining in isolated chondrocytes in the hypocellular area of articular cartilage.
我们采用抗S-100蛋白α和β亚基的单克隆抗体，运用抗生物素蛋白-生物素复合物法，研究了人关节软骨中S-100蛋白α和β亚基的免疫组化分布。在正常关节软骨的表层、中层和深层区域的软骨细胞中均检测到了两个亚基的免疫染色。在基质中，只有表层区域呈阳性染色。在关节炎关节中，我们在关节软骨细胞增多区域的成簇软骨细胞中检测到强烈的免疫染色，而在细胞减少区域的单个软骨细胞中免疫染色较弱或无免疫染色。
