Hou Zhe, Xiang Maolong, Chen Nuoya, Cai Xiao, Zhang Bo, Luo Rifang, Yang Li, Ma Xiaoyi, Zhou Lifeng, He Fugui, Yu Hongchi, Wang Yunbing
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
College of Life Sciences, Sichuan University, Chengdu 610064, China.
Regen Biomater. 2021 May 28;8(3):rbab017. doi: 10.1093/rb/rbab017. eCollection 2021 Jun.
Due to its good biocompatibility and degradability, magnesium alloy (Mg alloy) has shown great promise in cardiovascular stent applications. Rapid stent re-endothelialization is derived from migrated and adhered endothelial cells (ECs), which is an effective way to reduce late thrombosis and inhibit hyperplasia. However, fundamental questions regarding Mg alloy affecting migration and adhesion of ECs are not fully understood. Here, we evaluated the effects of Mg alloy on the ECs proliferation, adhesion and migration. A global gene expression profiling of ECs co-culturing with Mg alloy was conducted, and the adhesion- and migration-related genes were examined. We found that Mg alloy had no adverse effects on ECs viability but significantly affected ECs migration and adhesion. Co-cultured with Mg alloy extract, ECs showed contractive adhesion morphology and decreased motility, which was supported by the down-regulation of adhesion-related genes ( and ) and migration-related genes ( and ). Accordingly, the re-endothelialization of Mg alloy stent was inhibited . Our results may provide new inspiration for improving the broad application of Mg alloy stents.
由于其良好的生物相容性和可降解性，镁合金在心血管支架应用中显示出巨大的潜力。快速的支架再内皮化源于迁移和黏附的内皮细胞（ECs），这是减少晚期血栓形成和抑制增生的有效方法。然而，关于镁合金影响ECs迁移和黏附的基本问题尚未完全了解。在此，我们评估了镁合金对ECs增殖、黏附和迁移的影响。对与镁合金共培养的ECs进行了全基因组表达谱分析，并检测了与黏附和迁移相关的基因。我们发现镁合金对ECs活力没有不利影响，但显著影响ECs的迁移和黏附。与镁合金提取物共培养时，ECs表现出收缩性黏附形态且运动性降低，这得到了黏附相关基因（和）和迁移相关基因（和）下调的支持。相应地，镁合金支架的再内皮化受到抑制。我们的结果可能为改善镁合金支架的广泛应用提供新的启示。
