Park M S, Subbiah M T
Department of Internal Medicine, University of Cincinnati, OH.
Am J Clin Nutr. 1988 Oct;48(4):963-9. doi: 10.1093/ajcn/48.4.963.
Effects of human or rabbit milk on cholesterol content, incorporation of [14C]oleate, and DNA synthesis were investigated in organ cultures of aorta from fetal and suckling rabbits. Human skim milk (50 mL/L) in organ culture decreased content (mumol/g protein) of aortic total cholesterol (control: 162.9 +/- 24.6 milk: 117.6 +/- 4.9) with significant decrease in cholesteryl esters (control: 16.5 +/- 5.1, milk: 2.3 +/- 0.5). The effect was observed in lipoprotein (total cholesterol: 120.2 +/- 8.8) and lipoprotein-free (total cholesterol: 85.4 +/- 5.1) fractions with molecular weight greater than 100,000. The human milk fraction with molecular weight greater than 100,000 was also the most active in promoting [3H]thymidine incorporation into DNA in fetal aorta. Although milk stimulated the incorporation of [14C] oleate into triglycerides (control: 38.8 +/- 2.5%, milk: 82.1 +/- 4.2%), decreased incorporation to phospholipids (control: 55.0 +/- 3.0%, milk: 11.3 +/- 1.8%) was observed. These studies suggest that milk contains factors influencing aortic lipid metabolism during development.
在胎儿和哺乳期兔子的主动脉器官培养物中，研究了人乳或兔乳对胆固醇含量、[14C]油酸掺入以及DNA合成的影响。器官培养中加入人脱脂乳（50 mL/L）可降低主动脉总胆固醇含量（微摩尔/克蛋白质）（对照组：162.9±24.6，乳组：117.6±4.9），胆固醇酯显著降低（对照组：16.5±5.1，乳组：2.3±0.5）。在分子量大于100,000的脂蛋白（总胆固醇：120.2±8.8）和无脂蛋白（总胆固醇：85.4±5.1）组分中观察到了这种作用。分子量大于100,000的人乳组分在促进[3H]胸腺嘧啶核苷掺入胎儿主动脉DNA方面也最具活性。尽管乳促进了[14C]油酸掺入甘油三酯（对照组：38.8±2.5%，乳组：82.1±4.2%），但观察到其掺入磷脂减少（对照组：55.0±3.0%，乳组：11.3±1.8%）。这些研究表明，乳中含有影响发育过程中主动脉脂质代谢的因子。
