Kim Yi Sook, Han Dohyun, Mo Ji Hun, Kim Yong Min, Kim Dae Woo, Choi Hyo Guen, Park Jong Wan, Shin Hyun Woo
Obstructive Upper airway Research (OUaR) Laboratory, Department of Pharmacology, Seoul National University College of Medicine, Seoul, Korea.
Department of Biomedical Sciences, Seoul National University Graduate School, Seoul, Korea.
Allergy Asthma Immunol Res. 2021 Jul;13(4):589-608. doi: 10.4168/aair.2021.13.4.589.
Chronic rhinosinusitis (CRS) is a heterogeneous chronic inflammatory condition of the paranasal sinuses and nasal passages. Although antibiotics are used to reduce inflammation or to treat an episode of acute rhinosinusitis, their effects on the nasal environment and host response in CRS is unclear.
We analyzed the effects of antibiotics on the nasal microbiome and secreted proteome in CRS using multi-omic analysis. Nasal secretions were collected from 29 controls, 30 CRS patients without nasal polyps (NP), and 40 CRS patients with NP. A total of 99 subjects were divided into 2 groups that included subjects who had taken antibiotics 3 months prior to sampling and those who had not. We performed 16S ribosomal DNA sequence analyses and Orbitrap mass spectrometry-based proteomic analyses. Spearman correlation was used to assess the correlations between the nasal microbiome and secreted proteome.
The associations between the nasal microbiome and secreted proteome were noted in subjects who had used antibiotics. Antibiotics could have stronger effects on their associations in patients with CRS with NP than in those without. It remains unknown whether these holistic changes caused by antibiotics are beneficial or harmful to CRS, however, the associations could be differentially affected by disease severity.
These findings provide new insight into the nasal environment and the host response in CRS.
慢性鼻-鼻窦炎（CRS）是鼻窦和鼻腔通道的一种异质性慢性炎症性疾病。尽管抗生素被用于减轻炎症或治疗急性鼻-鼻窦炎发作，但其对CRS患者鼻腔环境和宿主反应的影响尚不清楚。
我们使用多组学分析方法，分析了抗生素对CRS患者鼻腔微生物群和分泌蛋白质组的影响。收集了29名对照者、30名无鼻息肉（NP）的CRS患者和40名有NP的CRS患者的鼻腔分泌物。总共99名受试者被分为两组，一组包括在采样前3个月服用过抗生素的受试者，另一组包括未服用过抗生素的受试者。我们进行了16S核糖体DNA序列分析和基于Orbitrap质谱的蛋白质组学分析。采用Spearman相关性分析评估鼻腔微生物群与分泌蛋白质组之间的相关性。
在使用过抗生素的受试者中，观察到鼻腔微生物群与分泌蛋白质组之间存在关联。抗生素对有NP的CRS患者的这种关联的影响可能比对无NP的患者更强。然而，抗生素引起的这些整体变化对CRS是有益还是有害尚不清楚，不过这些关联可能会受到疾病严重程度的不同影响。
这些发现为CRS患者的鼻腔环境和宿主反应提供了新的见解。
