Second Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, P.R. China.
Department of Hematology, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong 510630, P.R. China.
Mol Med Rep. 2021 Sep;24(3). doi: 10.3892/mmr.2021.12263. Epub 2021 Jul 2.
Intracerebral hemorrhage (ICH) can stimulate neural regeneration, promoting tissue repair and recovery of nerve function. Tongfu Xingshen capsule (TXC) is a Chinese medicinal formula used to treat ICH and has been shown to protect brain tissue and improve nerve function in clinical studies. However, the effect of TXC on endogenous neural stem cells (NSCs) remains elusive. To explore the mechanisms underlying TXC action, a rat model of ICH was established. The effects of TXC on the proliferation and differentiation of NSCs were assessed in the subventricular zone (SVZ). TXC significantly improved nerve function defects, decreased brain water content and restored blood‑brain barrier integrity. Additionally, BrdU labeling showed that both high and low doses of TXC significantly increased the proportion of actively cycling NSCs positive for Nestin and glial fibrillary acidic protein, but did not affect the proliferation rates of NeuN‑positive neurons. Finally, TXC also upregulated the mRNA levels of brain‑derived neurotrophic factor and its receptor, TrκB, in affected brain tissues. Taken together, TXC accelerated neural repair and functional recovery after brain injury by potentially enhancing the proliferation and differentiation of endogenous NSCs into astroglial cells in the SVZ area.
脑出血 (ICH) 可刺激神经再生，促进组织修复和神经功能恢复。通腑醒神胶囊 (TXC) 是一种用于治疗 ICH 的中药方剂，临床研究表明其具有保护脑组织和改善神经功能的作用。然而，TXC 对内源性神经干细胞 (NSCs) 的影响尚不清楚。为了探讨 TXC 的作用机制，建立了 ICH 大鼠模型。评估了 TXC 对侧脑室下区 (SVZ) 中 NSCs 增殖和分化的影响。TXC 显著改善了神经功能缺陷，降低了脑含水量并恢复了血脑屏障的完整性。此外，BrdU 标记显示，高、低剂量的 TXC 均显著增加了巢蛋白和胶质纤维酸性蛋白阳性的活跃细胞周期 NSCs 的比例，但不影响神经元的增殖率。最后，TXC 还上调了受影响脑组织中脑源性神经营养因子及其受体 TrkB 的 mRNA 水平。综上所述，TXC 通过潜在地增强 SVZ 区内源性 NSCs 向星形胶质细胞的增殖和分化，加速了脑损伤后的神经修复和功能恢复。
