Pillai M R, Balaram P, Abraham T, Padmanabhan T K, Nair M K
Division of Immunology, Regional Cancer Centre, Trivandrum, India.
Am J Reprod Immunol Microbiol. 1988 Apr;16(4):159-62. doi: 10.1111/j.1600-0897.1988.tb00188.x.
Peripheral blood lymphocytes from 100 patients with various stages and histological types of squamous cell carcinoma of the uterine cervix were assayed for natural killer (NK) cell activity against K562 cells using the single cell cytotoxicity assay in agarose. The effect of autologous serum on NK cell activity and a quantitation of soluble antigen-antibody complexes was also carried out. The cancer patients showed reduced NK activity compared with normal controls, the reduction increasing with tumor load. Another observation was that patients with poorly differentiated tumours showed lower levels of NK activity. Addition of autologous serum resulted in further depression of NK activity in all groups of patients. An increase in circulating immune complexes was also evident in all groups of patients. Here again increase in tumor load and poorly differentiated tumors showed the highest levels. The results point toward a possible disturbance in NK cell activity that could be further depressed by autologous serum factors.
采用琼脂糖单细胞细胞毒性试验，对100例不同分期和组织学类型的子宫颈鳞状细胞癌患者的外周血淋巴细胞进行了针对K562细胞的自然杀伤（NK）细胞活性检测。同时还研究了自体血清对NK细胞活性的影响以及可溶性抗原-抗体复合物的定量分析。与正常对照组相比，癌症患者的NK活性降低，且随着肿瘤负荷的增加而降低。另一项观察结果是，肿瘤分化差的患者NK活性水平较低。添加自体血清导致所有患者组的NK活性进一步降低。所有患者组中循环免疫复合物也明显增加。同样，肿瘤负荷增加和分化差的肿瘤中循环免疫复合物水平最高。结果表明NK细胞活性可能存在紊乱，而自体血清因子可能会进一步抑制其活性。
