Vaquer S, Jordá J, López de la Osa E, Alvarez de los Heros J, López-García N, Alvarez de Mon M
Service of Gynecology, Hospital General de Guadalajare.
Gynecol Oncol. 1990 Jan;36(1):90-2. doi: 10.1016/0090-8258(90)90114-z.
Natural killer (NK) activity in peripheral blood mononuclear cells (PBMC) from women with squamous cell carcinoma of the uterine cervix (SCCUC) was studied. PBMC were obtained from 26 previously untreated patients with SCCUC at different stages of disease according to the FIGO classification (5 at stage I, 4 at stage II, 5 at stage III, 6 at stage IVa, and 6 belonging to stage IVb), as well as from 23 healthy age-matched women. These cells were used as effectors against 51Cr-labeled K-562 target cells in standard 4-hr cytotoxic assays. The NK activity displayed by PBMC from patients with local stages of the neoplasm (I, II, III, and IVa) was found to be similar to that exerted by PBMC from healthy controls (P greater than 0.05). Furthermore, there were no significant differences among mean values of NK activity in PBMC from women at these different stages of the disease (P greater than 0.05). However, the NK activity detected in the PBMC of patients with distant metastatic spread of the disease (stage IVb) was significantly depressed with respect to both controls and patients at any other earlier stage (P less than 0.05). We conclude that a decrease in the NK activity present in PBMC from women with SCCUC coincides with distant tumoral dissemination of the disease.
对子宫颈鳞状细胞癌（SCCUC）女性外周血单个核细胞（PBMC）中的自然杀伤（NK）活性进行了研究。根据国际妇产科联盟（FIGO）分类，从26例先前未经治疗的处于不同疾病阶段的SCCUC患者中获取PBMC（I期5例，II期4例，III期5例，IVa期6例，IVb期6例），以及从23名年龄匹配的健康女性中获取PBMC。在标准的4小时细胞毒性试验中，将这些细胞用作针对51Cr标记的K-562靶细胞的效应细胞。发现肿瘤局部阶段（I、II、III和IVa）患者的PBMC显示的NK活性与健康对照者的PBMC所发挥的NK活性相似（P>0.05）。此外，在疾病的这些不同阶段，女性PBMC中NK活性的平均值之间没有显著差异（P>0.05）。然而，疾病有远处转移扩散（IVb期）的患者的PBMC中检测到的NK活性相对于对照组和任何其他早期阶段的患者均显著降低（P<0.05）。我们得出结论，SCCUC女性的PBMC中存在的NK活性降低与疾病的远处肿瘤播散同时出现。
