Rahmadi Mahardian, Su'aida Nily, Yustisari Pratiwi, Agung Dewaandika Wahyu, Oktavia Hanaratri Elma, Andarsari Mareta Rindang, Aryani Toetik
Department of Clinical Pharmacy, Faculty of Pharmacy, University of Airlangga, Surabaya, Indonesia.
J Basic Clin Physiol Pharmacol. 2021 Jun 25;32(4):485-490. doi: 10.1515/jbcpp-2020-0424.
The association between stress and gastric ulcers has been well reported. This study is divided into two parts: the first part of this study is consisted of analyzing the effect of fluvoxamine administration by intracerebroventricular (ICV) and intraperitoneal (IP) injections on stress-induced gastric ulcers. The second part investigates the effect of ondansetron in influencing the protection of the gastric mucous by giving fluvoxamine to the mice before being induced with stress.
Water immersion restraint stress (WIRS) was used to induce stress. Fluvoxamine 50 and 100 mg/kg by IP injection, fluvoxamine 9.3 µg, and 18.6 µg by ICV injection 30 min before the induction of stress. Meanwhile, single drug and in combination administered to the mice, ondansetron 3 mg/kg was given by IP at 60 min, and fluvoxamine 50, 100 mg/kg orally at 30 min before stress induction.
The obtained results show fluvoxamine 50 and 100 mg/kg by IP, and fluvoxamine 18.6 µg by ICV had significantly reduced ulcer index with p<0.005, p<0.001, and p<0.005 while fluvoxamine 9.3 µg showed the insignificant result. Fluvoxamine 50 mg/kg, fluvoxamine 100 mg/kg, and ondansetron 3 mg/kg monotherapy have a significant reduction in ulcers with p<0.005, p<0.001, and p<0.05, while the combination drugs showed an insignificant reduction in ulcers.
Fluvoxamine with different administration routes and ondansetron monotherapy before stress reduce the occurrence of gastric ulcers, while the combination drugs did not increase the protective effect of the gastric mucosa.
压力与胃溃疡之间的关联已有充分报道。本研究分为两个部分：第一部分是分析通过脑室内（ICV）和腹腔内（IP）注射氟伏沙明对应激诱导的胃溃疡的影响。第二部分研究昂丹司琼在小鼠应激诱导前给予氟伏沙明时对胃黏膜保护作用的影响。
采用水浸束缚应激（WIRS）诱导应激。在应激诱导前30分钟，腹腔注射50和100mg/kg氟伏沙明、脑室内注射9.3μg和18.6μg氟伏沙明。同时，将单一药物及联合用药给予小鼠，在应激诱导前60分钟腹腔注射3mg/kg昂丹司琼，在应激诱导前30分钟口服50、100mg/kg氟伏沙明。
所得结果显示，腹腔注射50和100mg/kg氟伏沙明以及脑室内注射18.6μg氟伏沙明可显著降低溃疡指数，p<0.005、p<0.001和p<0.005，而9.3μg氟伏沙明结果不显著。50mg/kg氟伏沙明、100mg/kg氟伏沙明和3mg/kg昂丹司琼单药治疗可显著减少溃疡，p<0.005、p<0.001和p<0.05，而联合用药显示溃疡减少不显著。
不同给药途径的氟伏沙明和应激前昂丹司琼单药治疗可减少胃溃疡的发生，而联合用药并未增强胃黏膜的保护作用。
