在保守残基处具有额外功能基团的 8-17 DNA 酶的激活与催化金属离子有关。
Activation of 8-17 DNAzyme with extra functional group at conserved residues is related to catalytic metal ion.
机构信息
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology & Toxicology, Beijing 100850, China.
出版信息
Bioorg Med Chem Lett. 2021 Sep 15;48:128234. doi: 10.1016/j.bmcl.2021.128234. Epub 2021 Jun 29.
PMID:34214510
Abstract
In 8-17 DNAzyme, the end loop AGC is a highly conserved motif. Here we reported an activation approach by specific chemical modifications on A6 and C8 for more efficient Ca-mediated reaction. The importance of the end loop was further highlighted and its critical conservation broken for more powerful catalysts.
摘要
在 8-17 DNA 酶中，末端环 AGC 是一个高度保守的基序。在这里，我们报道了一种通过对 A6 和 C8 进行特定化学修饰来激活 Ca 介导反应的方法。进一步强调了末端环的重要性，并打破了其关键的保守性，以获得更强大的催化剂。
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