BCL(X)L 和 BCL2 增加乳腺癌细胞中线粒体动力学：来自功能和遗传研究的证据。

BCL(X)L and BCL2 increase mitochondrial dynamics in breast cancer cell: Evidence from functional and genetic studies.

机构信息

Department of Physiology & Medical Physics, Royal College of Surgeons in Ireland, Dublin 2, Ireland; Centre for System Medicine, Royal College of Surgeons in Ireland, Dublin 2, Ireland.

出版信息

Biochim Biophys Acta Mol Cell Res. 2021 Sep;1868(10):119095. doi: 10.1016/j.bbamcr.2021.119095. Epub 2021 Jun 30.

DOI:10.1016/j.bbamcr.2021.119095

PMID:34214511

Abstract

BCL2 family proteins are important regulators of mitochondrial outer membrane permeabilization (MOMP). In recent years, BCL2 family proteins have also been linked to the regulation of mitochondrial bioenergetics and dynamics. Given their overexpression in breast cancer cells, we sought to explore whether two key members of this family, BCL2 and BCL(X)L impacted on mitochondrial fusion/fission processes. By employing a single cell imaging and RNA sequencing we found that overexpression of BCL2 or BCL(X)L increases mitochondrial dynamics and alters the expression profile of genes involved in this process. Collectively, our data show that overexpression of BCL2 proteins regulates mitochondrial dynamics in breast cancer tumor cells.

摘要

BCL2 家族蛋白是调节线粒体外膜通透性（MOMP）的重要调控因子。近年来，BCL2 家族蛋白也与线粒体生物能量学和动力学的调节有关。鉴于它们在乳腺癌细胞中的过表达，我们试图探讨该家族的两个关键成员，BCL2 和 BCL(X)L 是否影响线粒体融合/裂变过程。通过单细胞成像和 RNA 测序，我们发现过表达 BCL2 或 BCL(X)L 会增加线粒体的动态变化，并改变参与该过程的基因的表达谱。总的来说，我们的数据表明，BCL2 蛋白的过表达调节乳腺癌肿瘤细胞中的线粒体动力学。