Faculty of Biosciences and Aquaculture, Nord University, Bodø, Norway.
The New Zealand Institute for Plant and Food Research, Nelson, New Zealand; Institut de Ciències del Mar, Spanish National Research Council (CSIC), Barcelona, Spain.
Genomics. 2021 Sep;113(5):2953-2964. doi: 10.1016/j.ygeno.2021.06.037. Epub 2021 Jun 30.
In vertebrates, the somatotropic axis comprising the pituitary gland, liver and muscle plays a major role in myogenesis. Its output in terms of muscle growth is highly affected by nutritional and environmental cues, and thus likely epigenetically regulated. Hydroxymethylation is emerging as a DNA modification that modulates gene expression but a holistic characterization of the hydroxymethylome of the somatotropic axis has not been investigated to date. Using reduced representation 5-hydroxymethylcytosine profiling we demonstrate tissue-specific localization of 5-hydroxymethylcytosines at single nucleotide resolution. Their abundance within gene bodies and promoters of several growth-related genes supports their pertinent role in gene regulation. We propose that cytosine hydroxymethylation may contribute to the phenotypic plasticity of growth through epigenetic regulation of the somatotropic axis.
在脊椎动物中，由垂体、肝脏和肌肉组成的躯体轴在肌发生中起着重要作用。其在肌肉生长方面的输出受到营养和环境线索的高度影响，因此可能受到表观遗传调控。羟甲基化作为一种调节基因表达的 DNA 修饰而出现，但迄今为止，尚未对躯体轴的羟甲基组进行全面表征。通过使用简化的代表性 5-羟甲基胞嘧啶分析，我们证明了 5-羟甲基胞嘧啶在单核苷酸分辨率下的组织特异性定位。它们在几个与生长相关基因的基因体和启动子中的丰度支持了它们在基因调控中的重要作用。我们提出，通过对躯体轴的表观遗传调控，胞嘧啶羟甲基化可能有助于生长的表型可塑性。
