Medical University of Plovdiv, Faculty of Pharmacy, Department of Chemical Sciences, 120 Buxton Bros Str., Plovdiv, Bulgaria.
İstanbul Yeni Yüzyıl Üniversitesi, Faculty of Pharmacy, 26 Yılanlı Ayazma Caddesi, İstanbul, Turkey.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Dec 5;262:120106. doi: 10.1016/j.saa.2021.120106. Epub 2021 Jun 25.
A simple, cheap, and environmentally friendly centrifuge-less cloud point extraction procedure was developed for the preconcentration of traces of Cu(II) before its spectrophotometric determination. It is based on a complexation reaction with the hydrophobic azo reagent 6-hexyl-4-(2-thiazolylazo)resorcinol (HTAR), in which a complex with a stoichiometric ratio of 1:1 and an absorption maximum at 535 nm is formed. The experimental conditions for Cu(II) determination were found: HTAR concentration (8 × 10 mol mL), mass fraction of the surfactant Triton X-114 (2.2%), pH (5.9, ammonium acetate buffer), and incubation time (10 min at 60 °C). The linear range, limit of detection, molar absorption coefficient and preconcentration factor were calculated to be 4.5-254 ng mL, 1.34 ng mL, 2.54 × 10 L mol cm, and 10, respectively. The effect of foreign ions was studied, and the proposed procedure was applied to the analysis of water samples and a saline solution for intravenous infusion.
一种简单、廉价且环保的无离心沉淀相萃取方法被开发用于痕量 Cu(II) 的预富集，然后再对其进行分光光度法测定。它基于与疏水性偶氮试剂 6-己基-4-(2-噻唑基偶氮)间苯二酚（HTAR）的络合反应，其中形成了摩尔比为 1:1 的络合物，最大吸收波长在 535nm。确定了 Cu(II)测定的实验条件：HTAR 浓度（8×10 mol mL）、表面活性剂 Triton X-114 的质量分数（2.2%）、pH 值（5.9，乙酸铵缓冲液）和孵育时间（60°C 时 10 分钟）。计算出的线性范围、检测限、摩尔吸光系数和预浓缩因子分别为 4.5-254ng mL、1.34ng mL、2.54×10 L mol cm 和 10。研究了外来离子的影响，并将提出的方法应用于水样和静脉输液用生理盐水的分析。
