VA Boston Healthcare System and the Dept. of Surgery, Brigham & Women's Hospital and Harvard Medical School, 1400 VFW PKW, West Roxbury (Boston), MA 02132, United States.
VA Boston Healthcare System and the Dept. of Surgery, Brigham & Women's Hospital and Harvard Medical School, 1400 VFW PKW, West Roxbury (Boston), MA 02132, United States.
Cell Calcium. 2021 Jul;97:102436. doi: 10.1016/j.ceca.2021.102436. Epub 2021 Jun 24.
The primary cilium maintains all of the necessary machinery to generate and interpret cAMP signals within its tiny volume, leading to the supposition that ciliary cAMP provides unique biological instructions separate from those derived from the rest of the cell body. A new paper by Truong et al. has used optogenetic and chemogenetic tricks to selectively manipulate cAMP signaling within the primary cilium. Their data show that ciliary but not cytosolic message preferentially regulates transcriptional activity via the hedgehog pathway leading to actions on zebrafish development. Computer modeling provides a rational explanation as to how the geometry of this organelle enables it to tune out cAMP signals from the cell body in order to pick up messages generated in the cilium.
纤毛维持着产生和解释 cAMP 信号的所有必要机制，其体积微小，因此人们推测，纤毛内的 cAMP 提供了独特的生物学指令，与源自细胞体其余部分的指令不同。特鲁昂等人的一篇新论文使用光遗传学和化遗传学技巧，选择性地在初级纤毛内操纵 cAMP 信号。他们的数据表明，纤毛内的 cAMP 信号而不是细胞质内的信号，通过 hedgehog 通路优先调节转录活性，从而影响斑马鱼的发育。计算机建模提供了一个合理的解释，说明这个细胞器的几何形状如何使其能够滤除来自细胞体的 cAMP 信号，以便接收纤毛中产生的信号。
